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| To investigate the effects of sleeve gastrectomy on cognitive function in offspring of obese rats based on autophagy mediated by BDNF/TrkB pathway |
| SUN Jiyou1, ZHAI Zhiwei1, REN Hongjie2, QIAO Luming3, DU Hengzhou4, LI Xiaojuan5 |
1. Department of Gastrointestinal Surgery, Laizhou People's Hospital, Yantai 261400, China;
2. Endoscopy Room, Laizhou People's Hospital, Yantai 264000, China;
3. Laboratory, Laizhou People's Hospital, Yantai 264000, China;
4. Department of Oncology, Laizhou People's Hospital, Yantai 264000, China;
5. Department of Endocrinology, Laizhou People's Hospital, Yantai 264000, China |
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Abstract Objective To investigate the effects of sleeve gastrectomy (SG) on cognitive function in offspring of obese rats based on autophagy mediated by BDNF/TrkB pathway. Methods Thirty clean female SD rats were selected and divided into control group, obesity group and SG group. The rats in each group were mated with healthy male rats fed ordinary diet. After the offspring of the mating rats in each group grew up to 3 weeks old. The cognitive function of offspring was detected by Morris water maze experiment. Nissl and H&E staining were used to detect the number of Nissl bodies in the hippocampus of rat offspring. The contents of total cholesterol (TG), triglyceride (TC) and free fatty acid (FFA) in serum of rat offspring in each group were detected by the kit. The protein levels of BDNF, TrkB, Beclin1 and LC3Ⅱ in offspring hippocampal tissues were detected by Western blotting, the levels of BDNF and TrkB in hippocampal tissues were detected by immunohistochemical staining, and the number of autophagosomes was detected by transmission electron microscopy. Results Compared with the control group, the progeny escape latency of the obese rats was prolonged and the frequency of crossing the platform was reduced, the number of Nissl bodies in hippocampus decreased, and the contents of TG, TC and FFA in serum increased, the protein levels of BDNF, TrkB, Beclin1 and LC3Ⅱ in hippocampus decreased, the number of autophagosomes decreased; Compared with the obese group, the offspring of SG group had shorter escape latency and increased platform crossing times, the number of Nissl bodies in hippocampus increased, and the contents of TG, TC and FFA in serum decreased, the protein levels of BDNF, TrkB, Beclin1 and LC3Ⅱ in hippocampus were increased, the number of autophagosomes increased. Conclusion SG can improve the cognitive function of offspring of obese rats by activating autophagy mediated by BDNF/TrkB pathway.
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Received: 08 January 2024
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2024, Vol. 21 
