尿石素A对肾癌786-O细胞的作用机制研究

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Abstract Objective To investigate the effect of UA on renal carcinoma 786-O cells and the potential mechanism. Methods Pubchem and Swiss target Prediction databases were used to search the structure and target of UA, and the promising targets of UA were picked out according to the probability of action marks, which ware greater than 0. The effects of UA on the viability, migration and invasion of 786-O cells were detected by CCK-8, flow cytometry, Transwell migration and invasion assay, respectively. The expression levels of P62, caspase3, lc3, Bcl-2 and Bax proteins were detected by Western blotting. Results Most of the predicted targets of UA were kinases and cyclins. UA inhibited the proliferation of 786-O cells in a concentration-dependent manner, as well as inhibited the expression of p62 and bcl2 in 786-O cells. And UA inhibited the migration and invasion ability. Moreover, the expression of caspase3, bax and lc3-II were down-regulated in 786-O cells. Conclusion UA can inhibit proliferation, induce apoptosis, inhibit migration and invasion in renal carcinoma cells. The mechanism may be related to inhibiting p62, bcl2 expression and promoting caspase3, bax and lc3-II expression in renal carcinoma cells.

Key words： urolithin renal cell carcinoma 786-O cells viability cell cycle apoptosis

Received: 14 July 2023

PACS:

R734.2

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	Articles by authors
	ZHANG Ying
	CHEN Qian
	SHI Hua
	GUO Jing
	XU Jinhong
	XU Shuxiong
	GONG Maodi

Cite this article:

ZHANG Ying,CHEN Qian,SHI Hua等. Biological effects of Urolithin A on renal carcinoma 786-O cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(1): 20-25.

URL:

http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I1/20

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