DFOG下调CD44表达抑制SKOV3卵巢癌干样细胞体外侵袭和迁移

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Abstract Objective To determine whether 7-Difluoromethoxyl-5, 4'-di-n-octyl genistein (DFOG) inhibits invasion and migration capabilities in SKOV3-derived ovarian cancer stem-like cells (OCSLCs) by downregulating the expression of CD44, a cancer stem cell surface marker. Methods CD44 gene was knocked down using the lentiviral delivery system. OCSLCs cells were treated with DFOG (0.1, 1.0 and 10 μM) or in combination with DFOG (10 μM) and shCD44. The expression levels of CD44 protein in OCSLCs infected with CD44 shRNA and treated with DFOG (0.1, 1.0 and 10.0 µM) were detected using western blot analysis. The cell invasion rates were determined using transwell invasion assay and cell migration rates were determined using Wound-Healing assay. Results CD44 knockdown significantly reduced the cell invasion rates and cell migration rates in OCSLCs cells. DFOG downregulated the expression of CD44 as well as attenuated cell invasion and cell migration capabilities in OCSLCs. Moreover, knockdown of CD44 gene collaborated with DFOG to inhibit the ability of cell invasion and cell migration in OCSLCs. Conclusion DFOG inhibits cell invasion and cell migration capabilities by downregulating CD44 expression in OCSLCs .

Key words： ovarian cancer cancer stem-like cells DFOG CD44 cell invasion cell migration

Received: 12 December 2022

PACS:

R737.31

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	Articles by authors
	LIN Jiazhi
	LIU Huiling
	DENG Yu'ao
	ZHANG Chen
	NING Yingxia

Cite this article:

LIN Jiazhi,LIU Huiling,DENG Yu'ao等. DFOG inhibits invasion and migration of SKOV3 ovarian cancer stem-like cells in vitro via down-regulating CD44 expression[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(2): 16-21.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I2/16

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