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| Inhibition of Migration and Invasionin SMMC-7721-derived Liver Cancer Stem-like Cells by FructusViticis Total Flavonoids via Blockage of FoxM1-MMP2 Signaling Axis |
| Objective To study the effect and mechanism of fructusviticis total flavonoids (FVTF) on the migration and invasion ability of SMMC-7721-derived liver cancer stem-like cells (LCSLCs). Methods The second generation of sphere forming cells (SFCs) obtained from SMMC-7721 cell line using suspension culture with the stem cell conditioned medium were used as LCSLCs. LCSLCswere treated with 0.1% DMSO or different concentrations of FVTF. The effects of FVTF on migration and invasion of LCSLCs was determined using cell migration assayand transwell invasion assay. Themechanism of FVTF action was#br# explored by analyzing the level of FoxM1 and MMP2 protein expression with Western bolt. |
| Medical College, Hunan Normal University |
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Abstract Objective To study the effect and mechanism of fructus viticis total flavonoids(FVTF) on the migration and invasion ability of SMMC-7721-derived liver cancer stem-like cells(LCSLCs). Methods The second generation of sphere forming cells(SFCs) obtained from SMMC-7721 cell line using suspension culture with the stem cell conditioned medium were used as LCSLCs. LCSLCs were treated with 0.1% DMSO or different concentrations of FVTF. The effects of FVTF on migration and invasion of LCSLCs was determined using cell migration assay and transwell invasion assay. The mechanism of FVTF action was explored by analyzing the level of FoxM1 and MMP2 protein expression with Western bolt. Results Compared with the control group,FVTF could downregulate the expression of FoxM1 and MMP2 protein and significantly inhibited the migration and invasion of LCSLCs in a concentration dependent manner. Conclusion FVTF effectively inhibited the migration and invasion ability of SMMC-7721-derived LCSLCs is associated with blockage of FoxM1–MM P2 signal axis.
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2018, Vol. 15 
