Diagnostic value of peripheral blood CXCL10-mRNA and QSOX1-mRNA expression levels combined with AFP detection in early hepatitis B virus-related hepatocellular carcinoma
LI Zeng, LI Haisha, LONG Liyuan, XIE Ya, LI Zenghui, LI Jinqiang
Department of Infection, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University, Changsha 410005
Abstract： Objective To explore the clinical application value of serum alpha-fetoprotein (AFP), CXCL10-mRNA, and QSOX1-mRNA detection in the diagnosis of hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC). Methods A total of 120 subjects were selected from hospitalized patients in the Department of Infectious Diseases and healthy individuals from the physical examination center at The First Hospital of Changsha from January 2022 to May 2023. The subjects included 50 patients with early HBV-related HCC, 40 patients with liver cirrhosis, and 30 healthy individuals. The expression levels of peripheral blood CXCL10-mRNA and QSOX1-mRNA were detected by RT-PCR, and the AFP level was measured using a radioimmunoassay. The differences in serum levels of CXCL10-mRNA, QSOX1-mRNA, and AFP among the three groups were compared. Receiver operating characteristic (ROC) curves were plotted, and the diagnostic value of CXCL10-mRNA, QSOX1-mRNA, and AFP markers in the early diagnosis of HBV-related HCC was analyzed. Results The positive rate of AFP detection in the peripheral blood of the hepatocellular carcinoma (HCC) group patients was higher than that in the liver cirrhosis group, and the difference was statistically significant (P<0.05). The expression levels of CXCL10-mRNA, QSOX1-mRNA, and AFP in the HCC group were higher than those in the liver cirrhosis group and the healthy control group (P<0.05). The serum levels of CXCL10-mRNA and QSOX1-mRNA in the liver cirrhosis group were significantly higher than those in the healthy control group (P<0.05). ROC curve analysis showed that the area under the curve (AUC) for CXCL10-mRNA, QSOX1-mRNA, and AFP in the early diagnosis of HBV-related HCC was 0.881, 0.790, and 0.814 respectively. The AUC for the combined detection of the three markers was 0.957, with a sensitivity and specificity for diagnosing HCC of 0.860 and 0.971, respectively, which were higher than those for the individual markers, indicating higher diagnostic performance. Conclusion The levels of CXCL10-mRNA and QSOX1-mRNA are highly expressed in the serum of patients with HBV-related HCC and cirrhosis. Combined detection of these markers with AFP can improve the early diagnostic efficiency of HBV-related HCC.
李曾, 李海莎, 龙丽媛, 谢雅, 黎增辉, 李金强. 外周血CXCL10-mRNA、QSOX1-mRNA表达水平联合AFP检测对HBV相关早期肝细胞癌的诊断价值[J]. 湖南师范大学学报(医学版), 2024, 21(5): 31-35.
LI Zeng, LI Haisha, LONG Liyuan, XIE Ya, LI Zenghui, LI Jinqiang. Diagnostic value of peripheral blood CXCL10-mRNA and QSOX1-mRNA expression levels combined with AFP detection in early hepatitis B virus-related hepatocellular carcinoma. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(5): 31-35.
2024, Vol. 21 
