DLAT: a novel prognostic biomarker and therapeutic target for hepatocellular carcinoma
CHEN Wei, YANG Jinhui
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital, Changsha 410005, China
Abstract：Objective The aim of this study is to investigate the expression and prognostic value of dihydrolipoamide S-acetyltransferase(DLAT) in hepatocellular carcinoma (HCC), and to provide a new biomarker for prognosis evaluation and drug therapy of HCC. Methods The expression, prognostic value and related biological function of DLAT in HCC were analyzed by bioinformatics. Then, the clinicopathological data of 50 HCC patients from Hunan Provincial People's Hospital were collected. The differential expression of DLAT in tumor tissues and adjacent tissues was analyzed by immunohistochemistry, and the prognostic value of DLAT was further analyzed. HCC cell lines HepG2, Huh-7, SMMC-7721 and human normal liver tissue cell line LO2 were cultured, and the expression of DLAT mRNA in each cell line was detected by q-PCR. HepG2 cells were transfected with si-DLAT (experimental group) and si-NC (control group), and CCK8 assay and flow cytometry were used to detect the effect of DLAT on cell proliferation and apoptosis. Results The expression of DLAT in HCC tissues is higher than that in normal tissues, and expression level of DLAT are positively correlated with poor prognosis. The high expression of DLAT in HCC is closely related to the level of immune cell infiltration and cell cycle regulatory genes. After silencing DLAT, the proliferation of HepG2 cells was significantly slower than that of the control group, and the proportion of apoptosis was higher than that of the control group. Conclusion DLAT can be used as a good prognostic marker for patients with HCC, and may become a new target for the treatment of HCC.
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2024, Vol. 21 
