腺病毒肺炎患儿干扰素-λ1表达水平与腺病毒载量的关系

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摘要 目的: 研究人腺病毒（human adenovirus,HAdV）感染后呼吸道上皮细胞中的λ1干扰素（interferon-λ1,IFN-λ1）的表达情况。方法: 选取2019年1―12月因腺病毒肺炎住院的143例患儿为HAdV组,另选取同期35例呼吸道病原结果均阴性者作对照组,采集鼻咽拭子提取核酸,用荧光定量 PCR 检测病毒载量、IFN-λ1 mRNA 表达水平和HAdV测序分型。结果: HAdV组、HAdV组中不同年龄段、单纯感染组、混合感染组、重症组、非重症组、HAdV-3组、HAdV-7 组IFN-λ1 mRNA的表达水平均低于对照组。重症组HAdV载量高于非重症组;HAdV组、重症组、HAdV-7组的IFN-λ1 mRNA的表达水平与病毒载量有相关性。结论: 儿童腺病毒肺炎病情严重程度与病毒载量相关,其IFN-λ1表达水平降低,提示IFN-λ1可能参与了腺病毒感染的疾病进程。

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	刘思兰
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关键词 ：儿童, 腺病毒肺炎, 干扰素-λ1

Abstract：Objective To study the expression of IFN-λ1 in respiratory epithelial cells infected with human adenovirus (HAdV). Methods 143 children hospitalized with adenovirus pneumonia from January to December, 2019 were selected as the HAdV group, and 35 patients with negative sputum culture and seven respiratory antigens were selected as the control group. Nasopharyngeal swabs were collected to extract nucleic acids, and the viral load, IFN-λ1mRNA expression level and HAdV sequencing typing were detected by fluorescence quantitative PCR. Results The expression levels of IFN-λ1mRNA in HAdV group and HAdV group at different ages, simple infection group, mixed infection group, severe group, non-severe group, HAdV-3 group and HAdV-7 group were lower than those in the control group (P<0.05). The HAdV load in severe group was higher than that in non-severe group (P<0.05). The expression level of IFN-λ1mRNA in HAdV group, severe group and HAdV-7 group was correlated with viral load (P<0.01). Conclusion The severity of adenovirus pneumonia in children is related to the viral load but not to the expression of IFN-λ1, and the expression level of IFN-λ1 is decreased, suggesting that IFN-λ1 may be involved in the disease process of adenovirus infection.

Key words： children adenovirus pneumonia interferon-λ1

收稿日期: 2023-11-01

中图分类号:

R725.6

基金资助:湖南省创新型省份建设专项（2020JJ8011）

通讯作者: 黄寒,E-mail：huanghanpd@126.com

引用本文:

刘思兰, 黄寒, 钟礼立, 彭力, 林琳, 魏桑子. 腺病毒肺炎患儿干扰素-λ1表达水平与腺病毒载量的关系[J]. 湖南师范大学学报(医学版), 2024, 21(3): 126-131. LIU Silan, HUANG Han, ZHONG Lili, PENG Li, LIN Lin, WEI Sangzi. Relationship between IFN -λ1 expression level and adenovirus load in children with adenovirus pneumonia. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(3): 126-131.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2024/V21/I3/126

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