慢性牙周炎患者血清Th17/Treg细胞因子表达水平及临床意义

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摘要 目的: 探讨慢性牙周炎（chronic periodontitis,CP）患者辅助性T细胞17（T helper cell 17,Th17）、调节性T细胞（T regulatory cells,Treg）及相关细胞因子的表达情况,并分析其临床意义。方法: 选取2020年1月—2022年6月在我院口腔科就诊的CP患者90例和牙周健康者45例,分为对照组（n=45）、轻度组（n=45）和中重度组（n=45）。采用流式细胞术检测外周血Th17和Treg水平,并计算Th17/Treg;采用酶联免疫吸附法检测血清白介素6（interleukin-6,IL-6）、白介素17（interleukin-17,IL-17）、白介素10（interleukin-10,IL-10）、转化生长因子-β（transforming growth factor-β,TGF-β）、CC型趋化因子受体6（CC chemokine receptor 6,CCR6）和CC型趋化因子20（CC chemokine ligand 20,CCL20）水平。采用Pearson相关系数法分析外周血细胞及相关细胞因子与牙周临床指标的相关性。结果: 轻度组和中重度组外周血Th17、Th17/Treg高于对照组,Treg低于对照组;中重度组外周血Th17、Th17/Treg高于轻度组,Treg低于轻度组。三组间血清细胞因子水平比较,差异均有统计学意义;中重度组IL-6、IL-17、CCR6和CCL20水平最高,轻度组次之,对照组最低;轻度组IL-10和TGF-β水平最高,对照组次之,中重度组最低。Pearson相关分析显示,Th17、Th17/Treg、IL-6、IL-17、CCR6、CCL20与龈沟探诊深度（probing depth,PD）、牙周附着丧失（attachment loss,AL）均呈正相关,Treg与PD、AL均呈负相关,而IL-10、TGF-β与PD、AL均无相关性。结论: CP患者外周血Th17/Treg细胞比例失衡,并伴随着相关细胞因子的异常表达,血清中IL-6、IL-17、CCR6和CCL20的浓度变化与CP严重程度密切相关,可能促进了CP的发展。

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	刘书钗
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关键词 ：慢性牙周炎, Th17, Treg, 细胞因子

Abstract：Objective To investigate the expression of T helper cell 17(Th17), T regulatory cells (Treg) and related cytokines in patients with chronic periodontitis (CP), and to analyze its clinical significance. Methods From January 2020 to June 2022, 90 CP patients and 45 periodontally healthy subjects were selected from the Department of Stomatology of our hospital. They were divided into control group (n= 45), mild group (n= 45) and moderate to severe group (n=45). The levels of Th17 and Treg in peripheral blood were detected by flow cytometry, and Th17/Treg was calculated. The levels of serum interleukin-6(IL-6), interleukin-17(IL-17), interleukin-10(IL-10), transforming growth factor-β (TGF-β), CC chemokine receptor 6(CCR6) and CC chemokine ligand 20(CCL20) were detected by enzyme-linked immunosorbent assay. The correlation between peripheral blood cells and related cytokines and periodontal clinical indicators were analyzed by Pearson correlation coefficient. Results The peripheral blood Th17 and Th17/Treg in the mild group and the moderate-severe group were higher than those in the control group, and Treg was lower than that in the control group. Th17 and Th17 / Treg in peripheral blood of moderate to severe group were higher than those in mild group, and Treg was lower than that in mild group. There were significant differences in serum cytokine levels among the three groups. The levels of IL-6, IL-17, CCR6 and CCL20 were the highest in the moderate and severe group, followed by the mild group, and the lowest in the control group. The levels of IL-10 and TGF-β were the highest in the mild group, followed by the control group, and the lowest in the moderate and severe group. Pearson correlation analysis showed that Th17, Th17/Treg, IL-6, IL-17, CCR6 and CCL20 were positively correlated with probing depth (PD) and attachment loss (AL). Treg was negatively correlated with PD and AL, while IL-10 and TGF-β were not correlated with PD and AL. Conclusion The imbalance of Th17/Treg cells in peripheral blood of CP patients is accompanied by abnormal expression of related cytokines. The changes of serum IL-6, IL-17, CCR6 and CCL20 concentrations are closely related to the severity of CP, which may promote the development of CP.

Key words： chronic periodontitis Th17 Treg cytokines

收稿日期: 2023-06-01

中图分类号:

R781

基金资助:河北省医药卫生科技项目（13010520190284）

通讯作者: 刘书钗,E-mail：jxfp2023@126.com

引用本文:

刘书钗, 王美, 张楠. 慢性牙周炎患者血清Th17/Treg细胞因子表达水平及临床意义[J]. 湖南师范大学学报(医学版), 2023, 20(4): 47-50. LIU Shuchai, WANG Mei, ZHANG Nan. The expression level and clinical significance of serum Th17 / Treg cytokines in patients with chronic periodontitis. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(4): 47-50.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I4/47

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