右美托咪定对内毒素急性肺损伤小鼠炎症反应的影响

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摘要 目的：研究右美托咪定（dexmedetomidine,DEX）对内毒素急性肺损伤小鼠的影响。方法：32只健康雄性C57BL/6小鼠,随机分为4组（8只/组）,假手术组（control组）、内毒素急性肺损伤组（LPS组）、高浓度DEX组（HD组）、低浓度DEX组（LD组）。Control组注射等容量生理盐水;LPS组腹腔注射LPS 3 mg/kg;DEX+LPS组在应用LPS前20分钟腹腔注射DEX（40 μg/kg或20 μg/kg）。病理组织切片检测肺组织炎症变化、测定肺湿重/干重比（W/D）;酶联免疫吸附法（ELISA）检测血清IL-6、IL-8和IL-1β含量的变化;qRT-PCR检测肺组织中miR-155、miR-146a的变化。结果：与control组比较,LPS组和DEX组肺组织炎症明显、肺W/D比值、IL-6、IL-8和IL-1β含量及miR-155表达水平升高、miR-146a表达水平降低;与LPS组比较,DEX组肺组织炎症明显好转、肺W/D比值、IL-6、IL-8和IL-1β含量及miR-155表达水平降低、miR-146a表达水平升高;与LD组比较HD组肺组织炎症明显好转、肺W/D比值、IL-6、IL-8和IL-1β含量及miR-155表达水平降低、miR-146a表达水平升高。结论：右美托咪定（DEX）可减轻内毒素急性肺损伤小鼠炎症反应,且可能与miR-146a和miR-155有关。

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	陈秋霞
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	陈安基
	段柏情

关键词 ：右美托咪定, 内毒素急性肺损伤, miR-146a, miR-155

Abstract：Objective Effect of dexmedetomidine (DEX) on endotoxin induced acute lung injury in mice. Methods 32 healthy male C57BL/6 mice were randomly divided into 4 groups (8 mice/group), sham operation group (control group), endotoxin acute lung injury group (LPS group), high concentration DEX group (HD group), low concentration DEX group (LD group). Control group was injected with saline of equal volume; LPS group was intraperitoneally injected with LPS 3 mg/kg; In DEX+LPS group, DEX (40 μg/kg or 20 μg/kg). The inflammatory changes of lung tissue and the ratio of wet weight to dry weight (W/D) were measured by pathological sections; Detection of serum IL-6, IL-8 and IL-1β by enzyme-linked immunosorbent assay (ELISA) Change of content; The changes of miR-155 and miR-146a in lung tissue were detected by qRT-PCR. Results Compared with control group, LPS group and DEX group had significant lung inflammation, lung W/D ratio, IL-6, IL-8, and IL-1β the content and expression level of miR-155 increased, while the expression level of miR-146a decreased (^*P<0.05); Compared with LPS group, lung inflammation, lung W/D ratio, IL-6, IL-8, and IL-1β in DEX group were significantly improved the content and expression level of miR-155 decreased, while the expression level of miR-146a increased (^#P<0.05); Compared with LD group, lung inflammation, lung W/D ratio, IL-6, IL-8, and IL-1β in HD group were significantly improved the content and expression level of miR-155 decreased, while the expression level of miR-146a increased (^$P<0.05). Conclusion Dextrmetomidine (DEX) can reduce the inflammatory reaction in mice with acute lung injury induced by endotoxin, which may be related to miR-146a and miR-155.

Key words： demetomidine acute lung injury caused by endotoxin miR-146a miR-155

收稿日期: 2023-01-29

中图分类号:

R363

基金资助:湖南省临床医疗技术创新引导项目（2021SK53202）,长沙市自然科学基金（kq2208477）

通讯作者: 陈安基,E-mail：7797936@qq.com;段柏情,E-mail：22757199@qq.com

引用本文:

陈秋霞, 刘流, 陈贵梅, 陈安基, 段柏情. 右美托咪定对内毒素急性肺损伤小鼠炎症反应的影响[J]. 湖南师范大学学报(医学版), 2023, 20(3): 24-27. CHEN Qiuxia, LIU Liu, CHEN Guimei, CHEN Anji, DUAN Boqing. Effect of dexmedetomidine on inflammatory response in mice with acute lung injury induced by endotoxin. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(3): 24-27.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I3/24

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