CircRNA_0629在胆囊癌患者中的临床意义及其与预后的关系

摘要
图/表
参考文献
相关文章 (1)

全文: PDF (2601 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要 目的：明确环状RNA 0629（circRNA_0629）在胆囊癌（gaubladder carcinoma,GBC）患者中的临床意义及其与预后的关系。方法：采用生物信息学分析GSE100363与GSE121714芯片结果,筛选出可能与GBC转移相关的circRNAs。收集110例GBC患者的癌组织与癌旁组织,采用实时荧光定量PCR（qRT-PCR）验证circRNAs的表达水平。采用卡方检验分析circRNAs表达与临床病理特征的关系。采用卡普兰-迈耶（K-M）法和时序检验分析circRNAs表达与总体生存期（OS）的关系。采用Cox比例风险回归模型分析GBC预后的影响因素。采用受试者工作特征（ROC）曲线评估circRNAs诊断GBC肝转移和TNM分期的曲线下面积（AUC）值。结果：共筛选出3种circRNAs,分别为circRNA_049637、circRNA_402458、circRNA_0629。癌组织中circRNA_0629表达较癌旁组织升高。癌组织中circRNA_049637和circRNA_402458表达与癌旁组织比较,差异无统计学意义。circRNA_0629表达与肝转移以及TNM 分期相关。高circRNA_0629表达GBC患者1年和2年OS较低circRNA_0629表达GBC患者降低。高circRNA_0629表达、肝转移以及Ⅲ~Ⅳ期是GBC患者预后不良的独立危险因素。circRNA_0629对GBC肝转移和TNM分期具有较高诊断价值（AUC：0.929和0.835）。结论：circRNA_0629在GBC中表达上调,是预后不良的独立危险因素。circRNA_0629表达可作为协助评价GBC肝转移和TNM分期的一种新型标志物。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李吉滨
	姜世豪
	阳镇
	周宁

关键词 ：环状RNA 0629, 胆囊癌, 总体生存期, 肝转移, 标志物

Abstract：Objective: To determine the clinical significance of circular RNA 0629(circRNA_0629) in patients with gallbladder cancer (GBC) and its relationship with prognosis. Methods The results of GSE100363 and GSE121714 microarrays were analyzed using bioinformatics to screen for circRNAs that might be associated with GBC metastasis. Cancer tissues and paracancerous tissues from 110 GBC patients were collected, and the expression levels of circRNAs were verified by quantitative real-time PCR (qRT-PCR). The relationship between circRNAs expression and clinicopathological characteristics was analyzed by chi-square test. The association between circRNAs expression and overall survival (OS) was analyzed by Kaplan-Meyer (K-M) method and Long-Rank test. The factors that affecting prognosis of GBC were analyzed by Cox proportional risk regression model. The area under the curve (AUC) values of circRNAs for the diagnosis of GBC liver metastases and TNM staging were assessed by receiver operating characteristic (ROC) curves. Results A total of three circRNAs were screened, including circRNA_049637, circRNA_402458 and circRNA_0629. The expression of circRNA_0629 was higher in cancer tissues than that in paracancerous tissues. The differences of circRNA_049637 and circRNA_402458 expression in cancer tissues were not statistically significant when compared with paracancerous tissues. The expression of circRNA_0629 was correlated with liver metastasis and TNM staging. The 1- and 2-year OS were lower in GBC patients with high circRNA_0629 expression than those of GBC patients with low circRNA_0629 expression. High circRNA_0629 expression, liver metastasis and stage III~IV were independent risk factors for poor prognosis in GBC patients. circRNA_0629 had high diagnostic values for GBC liver metastases and TNM staging (AUC: 0.929 and 0.835). Conclusions circRNA_0629 expression is upregulated in GBC and is an independent risk factor for poor prognosis. circRNA_0629 expression may serve as a novel biomarker to assist in the evaluation of liver metastasis and TNM staging in GBC.

Key words： circRNA_0629 GBC OS liver metastasis biomarker

收稿日期: 2023-01-18

中图分类号:

R735.8

基金资助:湖南省自然科学基金科卫联合项目（2022JJ70100）,长沙市科技计划项目（kq2004117）

通讯作者: 周宁,E-mail：zhouning565@hunnu.edu.cn

引用本文:

李吉滨, 姜世豪, 阳镇, 周宁. CircRNA_0629在胆囊癌患者中的临床意义及其与预后的关系[J]. journal1, 2023, 20(2): 93-100. LI Jibin, JIANG Shihao, YANG Zhen, ZHOU Ning. Clinical significance of circRNA_0629 in patients with gallbladder cancer and its relationship with prognosis. journal1, 2023, 20(2): 93-100.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I2/93

[1] Wu X, Li B, Zheng C, et al.Incidental gallbladder cancer after laparoscopic cholecystectomy: incidence, management, and prognosis[J]. Asia Pac J Clin Oncol, 2020, 16(3): 158-164.
[2] Siegel RL, Miller KD, Fuchs HE, et al.Cancer statistics, 2022[J]. CA Cancer J Clin, 2022, 72(1): 7-33.
[3] Shen H, He M, Lin R, et al.PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway[J]. J Exp Clin Cancer Res, 2019, 38(1): 247.
[4] Patop IL, Wust S, Kadener S.Past, present, and future of circRNAs[J]. EMBO J, 2019, 38(16): e100836.
[5] Yu T, Wang Y, Fan Y, et al.CircRNAs in cancer metabolism: a review[J]. J Hematol Oncol, 2019, 12(1): 90.
[6] Kristensen LS, Jakobsen T, Hager H, et al.The emerging roles of circRNAs in cancer and oncology[J]. Nat Rev Clin Oncol, 2022, 19(3): 188-206.
[7] Huang X, He M, Huang S, et al.Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription[J]. Mol Cancer, 2019, 18(1): 166.
[8] Wang S, Zhang Y, Cai Q, et al.Circular RNA FOXP1 promotes tumor progression and Warburg effect in gallbladder cancer by regulating PKLR expression[J]. Mol Cancer, 2019, 18(1): 145.
[9] Wang S, Tong H, Su T, et al.CircTP63 promotes cell proliferation and invasion by regulating EZH2 via sponging miR-217 in gallbladder cancer[J]. Cancer Cell Int, 2021, 21(1): 608.
[10] Wang S, Su TT, Tong H, et al.CircPVT1 promotes gallbladder cancer growth by sponging miR-339-3p and regulates MCL-1 expression[J]. Cell Death Discov, 2021, 7(1): 191.
[11] Fares J, Fares MY, Khachfe HH, et al.Molecular principles of metastasis: a hallmark of cancer revisited[J]. Signal Transduct Target Ther, 2020, 5(1): 28.
[12] Wang X, Li J, Bian X, et al.CircURI1 interacts with hnRNPM to inhibit metastasis by modulating alternative splicing in gastric cancer[J]. Proc Natl Acad Sci U S A, 2021, 118(33): e2012881118.
[13] Chen J, Wu Y, Luo X, et al.Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer[J]. Theranostics, 2021, 11(15): 7507-7526.
[14] Zhang W, Liu T, Li T, et al.Hsa_circRNA_102002 facilitates metastasis of papillary thyroid cancer through regulating miR-488-3p/HAS2 axis[J]. Cancer Gene Ther, 2021, 28(3-4): 279-293.
[15] Zhao C, Sun J, Dang Z, et al.Circ_0000775 promotes the migration, invasion and EMT of hepatic carcinoma cells by recruiting IGF2BP2 to stabilize CDC27[J]. Pathol Res Pract, 2022, 235: 153908.
[16] Mao W, Deng F, Wang D, et al.Treatment of advanced gallbladder cancer: A SEER-based study[J]. Cancer Med, 2020, 9(1): 141-150.
[17] Yin G, Zheng S, Shan Y, et al.Circulating circRNA Has_Circ_0141633 Serves as a Potential Biomarker for Gastric Cancer[J]. Clin Lab, 2020, 66(9): 191234.
[18] Yao L, Xie Y.Down-regulation of hsa_circ_0006470 predicts tumor invasion: A new biomarker of gastric cancer[J]. J Clin Lab Anal, 2021, 35(8): e23879.
[19] Wang X, Lin YK, Lu ZL, et al.Circular RNA circ-MTO1 serves as a novel potential diagnostic and prognostic biomarker for gallbladder cancer[J]. Eur Rev Med Pharmacol Sci, 2020, 24(16): 8359-8366.
[20] Cheng Y, Su Y, Wang S, et al.Identification of circRNA-lncRNA-miRNA-mRNA Competitive Endogenous RNA Network as Novel Prognostic Markers for Acute Myeloid Leukemia[J]. Genes (Basel), 2020, 11(8): 868.
[21] Liang HX, Li YH.MiR-873, as a suppressor in cervical cancer, inhibits cells proliferation, invasion and migration via negatively regulating ULBP2[J]. Genes Genomics, 2020, 42(4): 371-382.
[22] Gu G, Tian L, Herzog SK, et al.Hormonal modulation of ESR1 mutant metastasis[J]. Oncogene, 2021, 40(5): 997-1011.
[23] Li P, Cao G, Zhang Y, et al.FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors[J]. Aging (Albany NY), 2020, 12(1): 53-69.
[24] Herberts C, Murtha AJ, Fu S, et al.Activating AKT1 and PIK3CA Mutations in Metastatic Castration-Resistant Prostate Cancer[J]. Eur Urol, 2020, 78(6): 834-844.
[25] Imianowski CJ, Whiteside SK, Lozano T, et al.BACH2 restricts NK cell maturation and function, limiting immunity to cancer metastasis[J]. J Exp Med, 2022, 219(12): e20211476.