尿石素A对肾癌786-O细胞的作用机制研究

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Abstract Objective To investigate the effect of UA on renal carcinoma 786-O cells and the potential mechanism. Methods Pubchem and Swiss target Prediction databases were used to search the structure and target of UA, and the promising targets of UA were picked out according to the probability of action marks, which ware greater than 0. The effects of UA on the viability, migration and invasion of 786-O cells were detected by CCK-8, flow cytometry, Transwell migration and invasion assay, respectively. The expression levels of P62, caspase3, lc3, Bcl-2 and Bax proteins were detected by Western blotting. Results Most of the predicted targets of UA were kinases and cyclins. UA inhibited the proliferation of 786-O cells in a concentration-dependent manner, as well as inhibited the expression of p62 and bcl2 in 786-O cells. And UA inhibited the migration and invasion ability. Moreover, the expression of caspase3, bax and lc3-II were down-regulated in 786-O cells. Conclusion UA can inhibit proliferation, induce apoptosis, inhibit migration and invasion in renal carcinoma cells. The mechanism may be related to inhibiting p62, bcl2 expression and promoting caspase3, bax and lc3-II expression in renal carcinoma cells.

Key words： urolithin renal cell carcinoma 786-O cells viability cell cycle apoptosis

Received: 14 July 2023

PACS:

R734.2

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	Articles by authors
	ZHANG Ying
	CHEN Qian
	SHI Hua
	GUO Jing
	XU Jinhong
	XU Shuxiong
	GONG Maodi

Cite this article:

ZHANG Ying,CHEN Qian,SHI Hua等. Biological effects of Urolithin A on renal carcinoma 786-O cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(1): 20-25.

URL:

http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I1/20

[1] BAI S, WU Y, YAN Y, et al.Construct a circRNA/miRNA/mRNA regulatory network to explore potential pathogenesis and therapy options of clear cell renal cell carcinoma[J]. Sci Rep, 2020, 10(1): 13659.
[2] MAKINO T, KADOMOTO S, IZUMI K, et al.Epidemiology and Prevention of Renal Cell Carcinoma[J]. Cancers (Basel), 2022, 14(16): 4059.
[3] BARATA PC, RINI BI.Treatment of renal cell carcinoma: Current status and future directions[J]. CA Cancer J Clin, 2017.67(6): 507-524.
[4] 杨韬, 封龙飞, 郭欢, 等. 双氢青蒿素通过自噬作用抗肿瘤的研究现状[J]. 中国临床药理学杂志, 2023, 39(15): 2271-2275.
[5] 孙菲, 袁庆, 张坚松. 芹菜素通过下调HK-2表达抑制非小细胞肺癌H460细胞糖酵解和球形成[J]. 湖南师范大学学报 (医学版), 2023, 20(1): 1-12.
[6] 刘丽华, 孙菲, 周志凌, 等. 芹菜素经上调miR148a-3p表达抑制MHCC97H细胞迁移和侵袭[J]. 湖南师范大学学报 (医学版), 2021, 18(06): 7-9.
[7] 张栖溶, 张莹. 中药大黄有效提取物大黄素诊治肿瘤的研究进展[J]. 标记免疫分析与临床, 2022, 29(10): 1784-1788.
[8] D'AMICO D, ANDREUX PA, VALDÉS P et al. Impact of the Natural Compound Urolithin A on Health, Disease, and Aging[J]. Trends Mol Med, 2021, 27(7): 687-699.
[9] CÁSEDAS G, LES F, CHOYA-FOCES C, et al. The Metabolite Urolithin-A Ameliorates Oxidative Stress in Neuro-2a Cells, Becoming a Potential Neuroprotective Agent[J]. Antioxidants (Basel), 2020, 9(2): 177.
[10] BELCARO G, SAGGINO A, CORNELLI U, et al.Improvement in mood, oxidative stress, fatigue, and insomnia following supplementary management with Robuvit®[J]. J Neurosurg Sci, 2018, 62(4): 423-427.
[11] SINGH R, CHANDRASHEKHARAPPA S, BODDULURI SR, et al.Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway[J]. Nat Commun, 2019, 10(1): 89.
[12] REN J, ZHANG Y.Targeting Autophagy in Aging and Aging-Related Cardiovascular Diseases[J]. Trends Pharmacol Sci, 2018, 39(12): 1064-1076.
[13] 刘国军. 黄绪群. 尿石素A对乳腺癌细胞MCF-7增殖、凋亡的影响及其机制研究[J]. 药物评价研究, 2020, 43(2): 221-225.
[14] STANISŁAWSKA IJ, GRANICA S, PIWOWARSKI JP, et al. The Activity of Urolithin A and M4 Valerolactone, Colonic Microbiota Metabolites of Polyphenols, in a Prostate Cancer In Vitro Model[J]. Planta Med, 2019, 85(2): 118-125.
[15] ZHANG W, CHEN JH, AGUILERA-BARRANTES I, et al.Urolithin A suppresses the proliferation of endometrial cancer cells by mediating estrogen receptor-α-dependent gene expression[J]. Mol Nutr Food Res, 2016, 60(11): 2387-2395.
[16] SAHASHI H, KATO A, YOSHIDA M, et al.Urolithin A targets the AKT/WNK1 axis to induce autophagy and exert anti-tumor effects in cholangiocarcinoma[J]. Front Oncol, 2022, 12: 963314.
[17] LIBERAL J, CARMO A, GOMES C, et al.Urolithins impair cell proliferation, arrest the cell cycle and induce apoptosis in UMUC3 bladder cancer cells[J]. Invest New Drugs, 2017, 35(6): 671-681.
[18] 田弥, 赵丽蓉, 兰昱, 等. 尿石素A抗癌作用及其机制研究进展[J]. 药物流行病学杂志, 2023, 32(2): 213-220.
[19] TANNIR NM, PAL SK, ATKINS MB.Second-Line Treatment Landscape for Renal Cell Carcinoma: A Comprehensive Review[J]. Oncologist, 2018, 23(5): 540-555.
[20] MAO W, WANG K, ZHANG H, et al.Sarcopenia as a poor prognostic indicator for renal cell carcinoma patients undergoing nephrectomy in China: A multicenter study[J]. Clin Transl Med, 2021, 11(1): e270.
[21] SÁNCHEZ-GONZÁLEZ C, CIUDAD CJ, IZQUIERDO-PULIDO M, et al. Urolithin A causes p21 up-regulation in prostate cancer cells[J]. Eur J Nutr, 2016, 55(3): 1099-1112.
[22] EL-WETIDY MS, AHMAD R, RADY I, et al.Urolithin A induces cell cycle arrest and apoptosis by inhibiting Bcl-2, increasing p53-p21 proteins and reactive oxygen species production in colorectal cancer cells[J]. Cell Stress Chaperones, 2021, 26(3): 473-493.
[23] 胡馨月, 彭书畅, 郭晓伟. 转录因子调控自噬的研究进展[J]. 湖南师范大学学报 (医学版), 2023, 20(02): 1-7.
[24] ISLAM MA, SOORO MA, ZHANG P Autophagic Regulation of p62 is Critical for Cancer Therapy[J]. Int J Mol Sci, 2018, 19(5): 1045.
[25] LI D, HE C, YE F, et al.p62 Overexpression Promotes Bone Metastasis of Lung Adenocarcinoma out of LC3-Dependent Autophagy[J]. Front Oncol, 2021, 11: 11609548.
[26] Li T, Jiang D, Wu K. p62 promotes bladder cancer cell growth by activating KEAP1/NRF2-dependent antioxidative response[J]. Cancer Sci, 2020, 111(4): 1156-1164.