TREM-1/NF-κB通路在二甲双胍调控小鼠急性肺损伤中的作用研究

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Abstract Objective This study was designed to examine the effect of metformin on triggering receptors expressed on myeloid cells-1（TREM-1）in a lipopolysaccharide（LPS）induced acute lung injury（ALI）mouse model and explore whether metformin utilizes TREM-1/NF- κB pathway to reduce ALI in mice. Methods First of all,an ALI mouse model was established by intraperitoneal injection of 10 mg/kg LPS,and intervention with metformin or TREM-1 receptor antagonist was used to examine the effect of metformin on TREM-1 expression in ALI mice. Total protein level,total cell and neutrophil count,lactate dehydrogenase（LDH）and myeloperoxidase（MPO）were determined in the lavage fluid（BALF）,and the wet/dry specific gravity of lung tissues were assessed. NF-κB and TREM-1 mRNA expression was assessed through Real time-PCR,while NF-κB,IL-18,IL-1β,TNF-α and TREM-1 protein expression levels were measured by Western blots. Results Our results showed that metformin reduced the expression of TREM-1 in the lung tissue of ALI mice. After antagonizing TREM-1,metformin could alleviate pathological damage,protein exudation,and neutrophil infiltration in lung tissue,and expression of NF-κB,IL-18,IL-1β,TNF-α was inhibited. Conclusion Metformin can reduce the expression of inflammatory factors,vascular permeability,and infiltration of inflammatory cells,thereby alleviating LPS-induced ALI,and its mechanism may be related to TREM-1/NF-κ B pathway.

Key words： acute lung injury metformin triggering receptors expressed on myeloid cells-1（TREM-1） nuclear factor-κB

Received: 25 September 2023

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R363

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	WEI Yunfu
	ZHOU Can
	LI Rui
	LUO Yutao
	HUANG Zhong'en
	REN Xiang
	SUN Guoying

Cite this article:

WEI Yunfu,ZHOU Can,LI Rui等. The role of TREM-1/NF- κB pathway in regulation of metformin on mouse acute lung injury[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(1): 8-14.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I1/8

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