IKBKB 基因敲除对胃癌细胞 NLRC5、NF-κB 表达以及生物学功能的影响

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Abstract Objective To investigate the relationship between the expression level of the IKBKB gene in gastric cancer cells and the activitiesy of NLRC5 and NF-κB. The effect of IKBKB knockdown on the biological function of gastric cancer cells was clarified. Methods The human gastric cancer SGC-7901 cell line was transfected directly with the plenti-ikkbkb-sgrna gene knockdownout plasmid. Western blot (WB) was used to detect the effect of Ikkbkb gene knockout and the expression levels of NLRC5 and NF-κB in the monoclonal cell line. Scratch test and Transwell migration assay were used to assess the migratory capacity of monoclonal cells, while flow cytometry was employed to measure apoptosis and cell cycle progression. The effect of monoclonal cells on tumor growth was observed in a tumorigenesis experiment. Immunohistochemical staining was used to evaluate the expression status of NLRC5, NF-κB, and IKBKB in clinical gastric cancer tissues and adjacent tissues. To clarify their existence, expression correlation, and clinical diagnostic value. Results After knocking out the IKBKB gene, the expression levels of NLRC5 and NF-κB in human gastric cancer SGC-7901 cells were significantly reduced, and the cell migration and invasion abilities were weakened. G1 phase arrest decreased the proliferation ability of SGC-79013 cells, and the total apoptosis rate of SGC-79013 cells significantly increased. The expression levels of NLRC5 and NF-κB were downregulated after IKBKB knockout, which impacted the growth and development of tumors. The expression levels of NLRC5, NF-κB, and IKBKB in gastric cancer tissues were higher than those in adjacent tissues. Additionally, the expression levels of NLRC5 and NF-κB were positively correlated with IKBKB. The binary logistic regression model was constructed with IKBKB, NLRC5, and NF-κB as explanatory variables. The diagnostic performance of the joint model was quantified using ROC analysis, which showed an AUC of 0.921 with a 95% confidence interval of 0.876-0.967. Conclusion IKBKB has a correlation with NLRC5 and NF-κB in gastric cancer cells, and synergistically promotes the occurrence and development of gastric cancer.

Key words： inhibitor of kappa light polypeptide gene enhancer in B-cells NLR family CARD domain containing 5 nuclear factor kappa-B gastric cancer cell biological function clinical diagnostic value

Received: 02 August 2023

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R735.2

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	Articles by authors
	ZHANG Gang
	GAO Tieming
	WU Yang
	ZHAO Zhi
	ZENG Zhixiang
	LI Zheli

Cite this article:

ZHANG Gang,GAO Tieming,WU Yang等. Effects of IKBKB gene knockdown on NLRC5 and NF-κB expression, as well as the biological function of gastric cancer cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(1): 1-8.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I1/1

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