外周血CXCL10-mRNA、QSOX1-mRNA表达水平联合AFP检测对HBV相关早期肝细胞癌的诊断价值

Abstract
Figure/Table
References
Related Citation (3)

Download: PDF (2173 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the clinical application value of serum alpha-fetoprotein (AFP), CXCL10-mRNA, and QSOX1-mRNA detection in the diagnosis of hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC). Methods A total of 120 subjects were selected from hospitalized patients in the Department of Infectious Diseases and healthy individuals from the physical examination center at The First Hospital of Changsha from January 2022 to May 2023. The subjects included 50 patients with early HBV-related HCC, 40 patients with liver cirrhosis, and 30 healthy individuals. The expression levels of peripheral blood CXCL10-mRNA and QSOX1-mRNA were detected by RT-PCR, and the AFP level was measured using a radioimmunoassay. The differences in serum levels of CXCL10-mRNA, QSOX1-mRNA, and AFP among the three groups were compared. Receiver operating characteristic (ROC) curves were plotted, and the diagnostic value of CXCL10-mRNA, QSOX1-mRNA, and AFP markers in the early diagnosis of HBV-related HCC was analyzed. Results The positive rate of AFP detection in the peripheral blood of the hepatocellular carcinoma (HCC) group patients was higher than that in the liver cirrhosis group, and the difference was statistically significant (P<0.05). The expression levels of CXCL10-mRNA, QSOX1-mRNA, and AFP in the HCC group were higher than those in the liver cirrhosis group and the healthy control group (P<0.05). The serum levels of CXCL10-mRNA and QSOX1-mRNA in the liver cirrhosis group were significantly higher than those in the healthy control group (P<0.05). ROC curve analysis showed that the area under the curve (AUC) for CXCL10-mRNA, QSOX1-mRNA, and AFP in the early diagnosis of HBV-related HCC was 0.881, 0.790, and 0.814 respectively. The AUC for the combined detection of the three markers was 0.957, with a sensitivity and specificity for diagnosing HCC of 0.860 and 0.971, respectively, which were higher than those for the individual markers, indicating higher diagnostic performance. Conclusion The levels of CXCL10-mRNA and QSOX1-mRNA are highly expressed in the serum of patients with HBV-related HCC and cirrhosis. Combined detection of these markers with AFP can improve the early diagnostic efficiency of HBV-related HCC.

Key words： chemokine ligand 10 quiescin sulfhydryl oxidase 1 alpha-fetoprotein hepatitis B virus-related hepatocellular carcinoma

Received: 14 April 2024

PACS:

R735.7

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Zeng
	LI Haisha
	LONG Liyuan
	XIE Ya
	LI Zenghui
	LI Jinqiang

Cite this article:

LI Zeng,LI Haisha,LONG Liyuan等. Diagnostic value of peripheral blood CXCL10-mRNA and QSOX1-mRNA expression levels combined with AFP detection in early hepatitis B virus-related hepatocellular carcinoma[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(5): 31-35.

URL:

http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I5/31

[1] BRAY F,LAVERSANNE M,SUNG H,et al.Global cancer statistics 2022:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2024,74(3):229-263.
[2] 李曾, 谢青, 李金强. QSOX1、CXCL10、β2GPI在乙型肝炎病毒相关性肝病患者中的表达及相关性研究[J]. 中国感染与化疗杂志, 2021, 21(05): 588-592.
[3] 张婧, 陈洁, 关贵文, 等. 肝细胞癌中趋化因子CXCL10及其受体CXCR3的表达及临床意义分析[J]. 北京大学学报 (医学版), 2019, 51(03): 402-408.
[4] 陈星, 戢敏, 喻雪琴, 等. 循环血CCL2、CCL5、CXCL10在HBV相关性肝病中表达水平的差异及相关性分析[J]. 川北医学院学报, 2019, 34(03): 354-358.
[5] 冯学敏, 郑文琪. 静息巯基氧化酶1在肿瘤微环境中的作用研究进展[J]. 生命的化学, 2020, 40(09): 1571-1575.
[6] TAL F, IRIS G, YOAV E, et al.Inhibition of fibroblast secreted QSOX1 perturbs extracellular matrix in the tumor microenvironment and decreases tumor growth and metastasis in murine cancer models[J]. Oncotarget, 2020, 11(4): 386-398.
[7] 国家卫生健康委. 原发性肝癌诊疗指南 (2022年版)[J]. 肿瘤综合治疗电子杂志, 2022, 8(02): 16-53.
[8] 林勇, 曾欣, 胡平方. 中国肝硬化临床诊治共识意见[J]. 临床肝胆病杂志, 2023, 39(09): 2057-2073.
[9] 尤红, 王福生, 李太生, 等. 慢性乙型肝炎防治指南 (2022年版)[J]. 实用肝脏病杂志, 2023, 26(03): 457-478.
[10] HAN B,ZHENG R,ZENG H,et al.Cancer incidence and mortality in China,2022[J]. Journal of the National Cancer Center,2024,4(1):47-53.
[11] DHANASEKARAN R, BANDOH S, ROBERTS LR. Molecular pathogenesis of hepatocellular carcinoma and impact of therapeutic advances[J]. F1000 Research, 2016, 5: F1000 Faculty Rev-879.
[12] 南月敏, 高沿航, 王荣琦, 等. 原发性肝癌二级预防共识 (2021年版)[J]. 临床肝胆病杂志, 2021, 37(03): 532-542.
[13] 邓文俊, 胡连涛, 赵彬男, 等. CXC趋化因子配体10对肝细胞癌SMMC-7721细胞增殖和迁移的影响及其机制[J]. 吉林大学学报 (医学版), 2023, 49(05): 1227-1233.
[14] JAVITT G, GROSSMAN-HAHAM I, ALON A, et al.cis-Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures[J]. Protein Sci, 2019, 28(1): 228-238.
[15] FIFIELD AL, HANAVAN PD, FAIGEL DO, et al.Molecular Inhibitor of QSOX1 Suppresses Tumor Growth In Vivo[J]. Molecular cancer therapeutics, 2020, 19(1): 112-122.
[16] 钟嘉宁. 巯基氧化酶1与HBV相关性肝病的相关性研究及其在肝癌生长中的作用初探[D]. 南宁: 广西医科大学, 2019.
[17] JIALEI S, CHENHAO Z, YUE Z, et al.Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation[J]. Redox Biology, 2021, 41101942-101942.
[18] 应慧文, 曹桂莲, 张弦. USP7和DNMT1在乙肝相关性肝癌中的表达及意义[J]. 南通大学学报 (医学版), 2023, 43(06): 531-535.
[19] 傅丽敏, 徐冬梅, 朱艳. AFP、PIVKA-Ⅱ和NLR在HBV-HCC中的诊断价值探讨[J]. 浙江临床医学, 2023, 25(10): 1532-1534.