山柰酚激活p38 MAPK信号通路促进人牙周韧带间充质干细胞的迁移和成骨细胞分化

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Abstract Objective To investigate the effect of kaempferol (KFR) in the migration and osteoblast differentiation of human periodontal ligament-derived mesenchymal stem cells (HPL-MSC) on the basis of p38 mitogen-activated protein kinase (MAPK) signaling pathway. Methods HPL-MSC cell line was cultured in vitro and divided into several groups: KFR group (0, 0.01, 0.1, 1, 10, 100 μmol/L KFR treatment) , p38 MAPK inhibitor SB203580 group (SB203580 group; 10 μmol/L SB203580 treatment) , 0.1 μmol/L KFR + SB203580 group (0.1 + SB203580 group; 0.1 μmol/L KFR and 10 μmol/L SB203580 co-treatment) , and drug treatment was for 24 h or 48 h. After treatment for 48 h, cells were incubated in the osteoblast inducible medium for 7 days. Cell migration and invasion ability was determined by scratch wound and Transwell assays, and osteoblast differentiation and p38 MAPK activation levels were evaluated by Western blotting. Cell viability was assessed by cell counting kit-8 (CCK8) . Results Compared with the 0 μmol/L KFR group, after 24 h treatment, cell viability of the 1 μmol/L KFR group was higher, while that of the 100 μmol/L KFR group was lower; after 48 h treatment, cell viability of the 0.01, 0.1 and 1 μmol/L KFR groups was higher, while that of the 100 μmol/L KFR group was lower. Therefore, the 0, 0.01, 0.1 and 1 μmol/L KFR groups were selected for cell function analysis. Compared with the 0 μmol/L KFR group, after 48 h treatment, cell migration rate and the relative expression levels of osteopontin (OPN) , bone salivary protein II (BSP-II) , bone morphogenetic protein 2 (BMP2) , bone alkaline phosphatase (ALP) , Runt-related transcription factor 2 (RUNX2) and osterix (OSX) in the 0.01, 0.1 and 1 μmol/L KFR group were higher, and invasive cells in the 0.1 and 1 μmol/L KFR groups were increased. In addition, relative expression level of phosphorylated p38 (p-p38) protein in the 0.01, 0.1 and 1 μmol/L KFR group was significantly higher than the 0 μmol/L KFR group, and that in the 0.1 μmol/L KFR group was higher than the 0.1 + SB203580 group, and that in the SB203580 group was lower than the 0.1 + SB203580 group. Compared with the 0.1 + SB203580 group, cell migration rate and the relative expression levels of OPN, BSP-II, BMP2, ALP, RUNX2 and OSX were higher in the 0.1 μmol/L KFR group, but lower in the SB203580 group, and invasive cells were higher in the 0.1 μmol/L KFR group, but lower in the SB203580 group. Conclusion KFR can promote the migration and osteogenic differentiation of HPL-MSC, which may be achieved by activating the p38 MAPK pathway.

Key words： kaempferol human periodontal ligament-derived mesenchymal stem cells osteoblastic differentiation migration p38 mitogen-activated protein kinase

Received: 10 May 2023

PACS:	R780.2
	R363

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	LI Zhaobao
	LI Zhaojing
	WANG Jing

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LI Zhaobao,LI Zhaojing,WANG Jing. Kaempferol promotes the migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells by activating p38 MAPK signaling pathway[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(4): 18-25.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I4/18

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