高同型半胱氨酸血症对紫癜性肾炎CBS<sup>+/-</sup>小鼠足细胞损伤的影响及机制

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Abstract Objective To study the effect and mechanism of homocysteinemia (HHcy) - induced podocyte injury in Henoch Schonlein purpura nephritis (HSPN) cystathionine -β- synthase (CBS) heterozygous mutation (CBS^+/-) mice. Methods HSPN model was established in wild type CBS (CBS^+/+) mice and CBS^+/-mice, then given conventional diet or high methionine diet respectively. The levels of serum Hcy, renal pathological and ultrastructural changes, serum creatinine (Scr), blood urea nitrogen (BUN), 24-hour urinary protein, the expression of podocin, nephrin and podocalyxin were compared. Results The renal pathological changes were found in the CBS^+/++HSPN group and the high methionine diet CBS^+/++HSPN group, and the levels of Scr, BUN and 24h urinary protein were higher than those in the conventional diet CBS^+/+group, but there was no significant difference in the renal pathological changes, Scr, BUN and 24h urinary protein levels between the high methionine diet CBS^+/++HSPN group and the conventional diet CBS^+/++ HSPN group. Renal pathological and ultrastructural changes were observed in conventional diet CBS^+/-+HSPN group, Scr, BUN and 24h urinary protein levels were higher than those of conventional diet CBS^+/- group, the expression of podocin, nephrin and podocalyxin were lower than those of conventional diet CBS^+/- group; compared with conventional diet CBS^+/-+HSPN group, renal athological and ultrastructural changes aggravated, Hcy, Scr, BUN and 24h urinary protein levels increased, the expression of podocin, nephrin and podocalyxin decreased of high methionine diet CBS^+/- group; compared with high methionine diet CBS^+/- group, renal athological and ultrastructural changes improved, Hcy, Scr, BUN and 24h urinary protein levels decreased, the expression of podocin, nephrin and podocalyxin increased in folic acid+ high methionine diet CBS^+/- group. Conclusion HHcy can promote renal pathological changes and podocyte injury in HSPN CBS^+/- mice, and inhibit the expression of transmembrane proteins podocin, nephrin and podocalyxin is a possible molecular mechanism.

Key words： Henoch Schonlein purpura Henoch Schonlein purpura nephritis hyperhomocysteinemia cystathionine β- synthase podocyte injury

Received: 22 July 2022

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R363

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	Articles by authors
	CHEN Leigang
	YANG Xiaojing
	FENG Dongmei
	WU Yuanhui
	WANG Shining
	GUO Baozhu

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CHEN Leigang,YANG Xiaojing,FENG Dongmei等. Effect and mechanism of hyperhomocysteinemia induced podocyte injury in Henoch Schonlein purpura nephritis CBS^+/- mice[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(4): 11-17.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I4/11

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