严重烫伤MODS小鼠胰岛β细胞功能下降与GLP-1/GLU比值相关性研究

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Abstract Objective To study the correlation between the reduction of islet β-cell function and the ratio of glucagon-like peptide 1/glucose (GLP-1/GLU) and explore its mechanism in severely scalded MODS mice so as to provide experimental basis for the prevention and treatment of the reduction of islet β-cell function in severely scalded MODS from a new perspective of improving the function of GLP-1 secretion in intestine. Methods 90 Kunming mice of Specific Pathogen Free (SPF) grade were randomly divided into three groups: 10 mice as the control group, 30 mice as simple scald group and 50 mice as MODS group. The mice in MODS group were made into MODS mice with 30% total body surface area Ⅲ degree scald and intraperitoneal injection of LPS. The mice in simple scald group were only inflicted with 30% total body surface area Ⅲ degree scald, and mice in the control group were sham scalded. Blood samples of mice in the control group were collected. At 1 day and 3 days after injury, 10 mice were randomly captured respectively from simple scald group and MODS group, and blood samples were collected. At 5 days after injury, blood samples of all surviving mice in scald group and MODS group were collected. The levels of GLU, plasma insulin, GLP-1 and diamine oxidase (DAO) of mice were determined. Homeostasis model assessment of β-cell function (HOMA-β) was applied, and then the index of islet β-cell function (HOMA-β) and the ratio of plasma GLP-1/GLU were calculated and observed. The correlation coefficient between HOMA-β and plasma GLP-1/GLU was calculated. The ileum tissues of mice were collected to observe the expression of GLP-1 by immunohistochemistry, and then the levels of intestinal GLP-1 secretion relative to blood glucose (intestinal GLP-1/GLU) were calculated and observed. The correlation coefficient between plasma GLP-1/GLU and intestinal GLP-1/GLU, and the correlation coefficient between intestinal GLP-1/GLU and plasma DAO were calculated. Data were statistically analyzed with one-way analysis of variance and Bonferroni test, Kruskal-Wallis and Bonferroni test, and Spearman rank correlation analysis. Results HOMA-β, plasma GLP-1/GLU and intestinal GLP-1/GLU of severely scalded MODS mice at 1, 3 and 5 days after injury were significantly lower than those of mice in the control group and simple scald group at the same time point (P<0.05). HOMA-β was positively correlated with plasma GLP-1/GLU of mice (r=0.685, P<0.001). Plasma GLP-1/GLU was positively correlated with intestinal GLP-1/GLU (r=0.851, P<0.001). Intestinal GLP-1/GLU was negatively correlated with DAO (r=-0.732, P<0.001). Conclusion The islet β-cell function and the ratio of GLP-1/GLU were decreased in severely scalded MODS mice. Insufficiency of plasma GLP-1 relative to blood glucose was correlated with the reduction of islet β-cell function. Intestinal damage led to relatively insufficient GLP-1 secretion. Protecting intestinal cells to increase the ratio of plasma GLP-1/GLU in severely scalded MODS mice can be used as an important measure to improve the reduction of islet β-cell function.

Key words： severe scalds MODS reduction of islet β-cell function GLP-1/GLU inflammatory cytokines

Received: 05 July 2022

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	LUO Yanji
	HUANG Zhihong
	XIAO Chuangqing
	YE Chunmei
	ZHONG Jie
	CAO Wenjing

Cite this article:

LUO Yanji,HUANG Zhihong,XIAO Chuangqing等. Study on the correlation between the reduction of islet β-cell function and the ratio of GLP-1/GLU in severely scalded MODS mice[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(1): 24-30.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I1/24

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