Preliminary study on STMN1 participating in radiotherapy resistance of NSCLC through apoptosis
OUYANG yunjie1, LIU Limin1, CHEN Nishichun3, YIN Xia2
1. Cadre Ward, The 921th Hospital of the Joint Logistics Support Force of PLA; 2. Department of Oncology, the 921th Hospital of the Joint Logistic Support Force of PLA; 3. Department of Gynaecologyand obsterics, the 921th Hospital of the Joint Logistic Support Force of PLA, Changsha, Hunan 410000
Abstract：Objective To investigate whether the expression of STMN1 is related to the prognosis of non-small cell lung cancer (NSCLC) and the effect of radiotherapy. Methods Immunohistochemical staining (IHC) was used to detect the expression of STMN1 in NSCLC and normal tissues. The correlation between STMN1 expression level and clinicopathological characteristics of patients was analyzed, and the correlation between STMN1 expression level and progression-free survival (PFS) was studied. STMN1 was identified as an independent prognostic factor for radiotherapy resistance in stage III-IV NSCLC by univariate and multivariate regression analysis. Constructing STMN1 overexpressing plasmids and interfering cell models of A549 and H1299 cells by a lentivirus infection method, verifying the expression of STMN1 and p-STMN1 by a protein immunoblot technology (Western-blot, WB), The effect of STMN1 on radiosensitivity of NSCLC cells was investigated by flow cytotoxicity analysis. The effect of STMN1 on cell invasion and migration was investigated by transwel1 invasion and migration assay. Results The expression level of STMN1 in NSCLC was significantly higher than that in normal lung. The expression level of STMN1 was significantly correlated with tumor differentiation and TNM staging in NSCLC patients, but not with age, sex and histopathological classification. The median PFS in STMN1 overexpression NSCLC was significantly lower than that in STMN1 underexpression. The local control rate of STMN1 overexpression NSCLC after 2 months of radiotherapy was significantly lower than that of STMN1 underexpression. Overexpression of STMN1 inhibited X-ray induced apoptosis of lung cancer cells. After STMN1 overexpression, the invasion and migration of A549 and H1299 cells were significantly enhanced. Conclusion The high expression of STMN is closely related to the low differentiation and TNM staging of tumor. Therefore, STMN1 can be used as an indicator of poor prognosis and resistance to radiotherapy in patients with NSCLC.
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