亚精胺通过抑制NF-κB/NLRP3炎症小体通路减轻LPS诱导的小鼠急性肺损伤

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摘要 目的: 本研究旨在观察亚精胺对脂多糖（LPS）诱导的急性肺损伤（ALI）小鼠NLRP3表达与活化的影响。方法: 采用小动物呼吸机检测亚精胺对ALI小鼠呼吸功能的影响;检测小鼠的生存率;采用肺组织病理评分评估亚精胺对ALI小鼠肺组织形态变化的影响;ELISA检测ALI小鼠支气管肺泡灌洗液（BALF）中IL -1β和IL-18的蛋白水平;qPCR检测小鼠肺组织IL -1β、IL-18、NLRP3、ASC及pro-caspase-1、NF-κB的表达;Western Blots检测小鼠肺组织IκB的表达。结果: 亚精胺可改善ALI小鼠的呼吸功能,提高生存率;减轻LPS诱导的肺病理损伤;降低ALI小鼠炎症因子IL-1β和IL-18的表达;减少ALI小鼠肺组织NLRP3、ASC、pro-caspase-1、NF-κB及IκB的表达。结论: 亚精胺可通过抑制ALI小鼠肺组织NF-κB的激活,使NLRP3炎症小体的表达及活化减少,从而抑制炎症因子的表达,最终达到减轻ALI的作用。本研究可为从调节内源性能量代谢角度,探索ALI的发生机制提供实验基础

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	罗宇涛
	粟青
	李锐
	杨睿
	郑通琦
	韦运富
	孙国瑛

关键词 ：亚精胺, 急性肺损伤, 脂多糖, 炎症小体

Abstract：Objective The purpose of this study was to observe the effect of spermidine on the expression and activation of NLRP3 in lipopolysaccharide (LPS) - induced acute lung injury (ALI) mice. Methods Effectof spermidine on respiratory function in ALI mice was detected by a small animal ventilator, and the survival rate of mice was detected. Pathological score were used to evaluate the effect of spermidine on the morphological changes of lung tissue in ALI mice, and levels of IL-1β and IL-18 protein levels in bronchoalveolar lavage fluid (BALF) of ALI mice were detectd by ELISA. The qPCR was used to detect the expression of IL-1β, IL-18, NLRP3, ASC, pro-caspase-1 and NF-κB in mouse lungs; IκB expression of mice was detected by Western blots. Results Spermidine could improve the respiratory function and survival rate of ALI mice, alleviate LPS induced lung pathological damage, and reduce IL-1β, IL-18, NLRP3, ASC, pro-caspase-1, NF-κB and IκB expression of ALI mice. Conclusions Spermidine may reduce the assembly and activation of NLRP3 inflammasome by inhibiting the activation of NF-κB in the lung tissue of ALI mice, thereby inhibit the expression of inflammatory factors, and ultimately lessening ALI. This study can provide an experimental basis for exploring the mechanism of ALI from the perspective of regulating endogenous energy metabolism.

Key words： spermidine acute lung injury lipopolysaccharide inflammatory corpuscle

收稿日期: 2022-12-11

中图分类号:

R363

基金资助:湖南省教育厅科学研究项目（21C0036）; 湖南省大学生创新创业训练计划项目（S202212652013）; 湖南省卫健委科研课题（202204075391,D202301019729）

通讯作者: 孙国瑛,E-mail：sunguoying1029@hunnu.edu.cn

作者简介: *为并列第一作者

引用本文:

罗宇涛, 粟青, 李锐, 杨睿, 郑通琦, 韦运富, 孙国瑛. 亚精胺通过抑制NF-κB/NLRP3炎症小体通路减轻LPS诱导的小鼠急性肺损伤[J]. 湖南师范大学学报(医学版), 2023, 20(1): 6-12. LUO Yutao, SU Qing, LI Rui, YANG Rui, ZHENG Tongqi, WEI Yunfu, SUN Guoying. Spermidine reduces LPS-induced ALI by inhibiting the NF-κB/ NLRP3 inflammasome pathway. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(1): 6-12.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I1/6

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