成人急性白血病血清MBL、MASP1和MASP2检测及其临床价值

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摘要 目的: 检测成人急性白血病血清甘露糖结合凝集素（Mannose-bindingLectin,MBL）、甘露糖结合凝集素相关丝氨酸蛋白酶-1（mannose-bindinglectinassociatedserineprotease-1,MASP1）和甘露糖结合凝集素相关丝氨酸蛋白酶-2（mannose-bindinglectinassociatedserineprotease-2,MASP2）水平并研究其临床价值。方法: 采用酶联免疫吸附试验（ELISA）检测成人急性白血病患者及正常健康人血清MBL、MASP1和MASP2水平,采用ROC曲线分析血清MBL、MASP1和MASP2在诊断成人急性白血病时的灵敏度、特异度、准确度。结果: 白血病组和对照组研究对象在MBL方面的差异无统计学意义,白血病组患者血清MASP1水平显著低于对照组健康人,而血清MASP2显著高于对照组健康人。复发白血病患者血清MBL水平显著低于初发白血病患者,MASP2显著高于初发白血病患者,而两组间血清MASP1差异无统计学意义。Logistics回归分析表明血清MBL、MASP1、MASP2水平和成人白血病均有一定的关联。MBL、MASP1联合MASP2诊断成人急性白血病灵敏度、特异性、准确度分别为0.854、0.810和0.664。结论: 成人白血病患者血清MASP1显著降低而 MASP2显著升高,MBL、MASP1及MASP2联合检测对成人白血病具有较高的诊断价值。

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	韦岩
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	李艳丽

关键词 ：成人急性白血病, MBL, MASP1, MASP2, 临床价值

Abstract：Objective To Detect the serum levels of MBL, MASP1 and MASP2 in adults with acute leukemia and study their clinical value Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum MBL, MASP1 and MASP2 in adult acute leukemia patients and normal healthy people, and receiver operating characteristic curve was used to analyze the sensitivity, specificity and accuracy of serum MBL, MASP1 and MASP2 in the diagnosis of adult acute leukemia. Results There was no significant difference in MBL between the leukemia group and the control group. The serum MASP1 level of the leukemia group was significantly lower than that of the control group, while the serum MASP2 level was significantly higher than that of the control group. People. The serum MBL level in patients with relapsed leukemia was significantly lower than that in patients with first-onset leukemia, and the level of MASP2 was significantly higher than that in patients with first-onset leukemia, while there was no significant difference in serum MASP1 between the two groups. Logistic regression analysis showed that serum levels of MBL, MASP1, MASP2 were associated with adult leukemia. The sensitivity, specificity and accuracy of MBL, MASP1 combined with MASP2 in the diagnosis of adult acute leukemia were 0.854, 0.810 and 0.664, respectively. Conclusion Serum MASP1 was significantly decreased while MASP2 was significantly increased in adult leukemia patients. The combined detection of MBL, MASP1 and MASP2 has a high diagnostic value for adult leukemia.

Key words： adult acute leukemia MBL MASP1 MASP2 clinical value

收稿日期: 2022-11-04

中图分类号:

R733.7

基金资助:山东省高等学校科技项目（J17KA227）

通讯作者: 韦岩,E-mail：weiyan20221026@163.com

引用本文:

韦岩, 李芳, 马壮壮, 赵晶, 张海燕, 李艳丽. 成人急性白血病血清MBL、MASP1和MASP2检测及其临床价值[J]. 湖南师范大学学报(医学版), 2023, 20(1): 44-47. WEI Yan, LI Fang, MA Zhuangzhuang, ZHAO Jing, ZHANG Haiyan, LI Yanli. Detection of Serum MBL, MASP1 and MASP2 in Adult Acute Leukemia and Its Clinical Value. HuNan ShiFan DaXue XueBao(Yi Xue Ban), 2023, 20(1): 44-47.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I1/44

[1] Cedzyński M, Świerzko AS.Components of the Lectin Pathway of Complement in Haematologic Malignancies[J]. Cancers (Basel), 2020, 12(7).
[2] Read BJ, Won L, Kraft JC, et al.Mannose-binding lectin and complement mediate follicular localization and enhanced immunogenicity of diverse protein nanoparticle immunogens[J]. Cell Rep, 2022, 38(2): 110217.
[3] Zhang CH, Xu ZQ, Peng LL, et al.Comprehensive analysis of the immune and prognostic implication of MASP1 in stomach adenocarcinoma[J]. Eur Rev Med Pharmacol Sci, 2022, 26(18): 6725-6741.
[4] 马尚, 尹颜军, 张鑫桐, 等. 非小细胞肺癌患者血清中MASP-2检测的临床价值[J]. 临床肺科杂志, 2020, 25(06): 908-911.
[5] 唐家泷, 贺明, 朱勇飞, 等. 汉族成年人慢性髓系白血病的环境危险因素分析[J]. 湖南师范大学学报 (医学版), 2022, 19(05): 138-142.
[6] Sokołowska A, Świerzko AS, Gajek G, et al.Associations of ficolins and mannose-binding lectin with acute myeloid leukaemia in adults[J]. Sci Rep, 2020, 10(1): 10561.
[7] 朱娟, 周英, 唐少华. MiR-186通过调控EZH2影响急性髓系白血病细胞增殖及凋亡[J]. 湖南师范大学学报 (医学版), 2021, 18(01): 1-4.
[8] 倪文娟, 云雁, 刘明君, 等. HPSE、MMP-2及MMP-9在急性白血病患者骨髓细胞中的表达及其相关性研究[J]. 中国全科医学, 2021, 24(S1): 133-135.
[9] Wei AH, Ribera JM, Larson RA, et al.Biomarkers associated with blinatumomab outcomes in acute lymphoblastic leukemia[J]. Leukemia, 2021, 35(8): 2220-2231.
[10] 王瑜, 黄纯兰, 鞠梅. 中老年急性白血病患者医院感染预测模型构建及验证[J]. 军事护理, 2023, 40(02): 33-37.
[11] Swierzko AS, Szala A, Sawicki S, et al.Mannose-Binding Lectin (MBL) and MBL-associated serine protease-2(MASP-2) in women with malignant and benign ovarian tumours[J]. Cancer Immunol Immunother, 2014, 63(11): 1129-1140.
[12] Xu CY, Dong JF, Chen ZQ, et al.MiR-942-3p Promotes the Proliferation and Invasion of Hepatocellular Carcinoma Cells by Targeting MBL2[J]. Cancer Control, 2019, 26(1): 1073274819846593.
[13] 李捷, 朱玲燕, 左大明, 等. 肝细胞肝癌患者血浆中甘露聚糖结合凝集素和甘露聚糖结合凝集素相关丝氨酸蛋白酶-2的表达水平[J]. 南方医科大学学报, 2017, 37(12): 1667-1672.
[14] Ytting H, Christensen IJ, Steffensen R, et al.Mannan-binding lectin (MBL) and MBL-associated serine protease 2(MASP-2) genotypes in colorectal cancer[J]. Scand J Immunol, 2011, 73(2): 122-127.
[15] Nevadunsky NS, Korneeva I, Caputo T, et al.Mannose-binding lectin codon 54 genetic polymorphism and vaginal protein levels in women with gynecologic malignancies[J]. Eur J Obstet Gynecol Reprod Biol, 2012, 163(2): 216-218.
[16] 阿衣夏木古丽·艾则孜, 刘洪柏, 马丽丽, 等. 血清甘露糖结合凝集素相关丝氨酸蛋白酶-2和半乳糖凝集素-3水平与老年肺腺癌患者淋巴结转移的相关性分析[J]. 中华实用诊断与治疗杂志, 2021, 35(12): 1198-1201.
[17] 李龙, 张阿龙, 李梦娇. 术前外周血LMR、MASP-2水平在Ⅲ期结肠癌患者预后评估中的价值研究[J]. 宁夏医学杂志, 2021, 43(05): 423-425.
[18] Takahashi M, Iwaki D, Kanno K, et al.Mannose-binding lectin (MBL) -associated serine protease (MASP) -1 contributes to activation of the lectin complement pathway[J]. J Immunol, 2008, 180(9): 6132-6138.
[19] Maestri CA, Nisihara R, Mendes HW, et al.MASP-1 and MASP-2 Serum Levels Are Associated With Worse Prognostic in Cervical Cancer Progression[J]. Front Immunol, 2018, 9: 2742.
[20] Ispasanie E, Muri L, Schubart A, et al.Alternative Complement Pathway Inhibition Does Not Abrogate Meningococcal Killing by Serum of Vaccinated Individuals[J]. Front Immunol, 2021, 12: 747594.
[21] Ytting H, Christensen IJ, Thiel S, et al.Pre- and postoperative levels in serum of mannan-binding lectin associated serine protease-2 -a prognostic marker in colorectal cancer[J]. Hum Immunol, 2008, 69(7): 414-420.
[22] Jalal PJ, King BJ, Saeed A, et al.Elevated serum activity of MBL and ficolin-2 as biomarkers for progression to hepatocellular carcinoma in chronic HCV infection[J]. Virology, 2019, 530: 99-106.
[23] Verma A, Matta A, Shukla NK, et al.Clinical significance of mannose-binding lectin-associated serine protease-2 expression in esophageal squamous cell carcinoma[J]. Int J Cancer, 2006, 118(12): 2930-2935.