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Quick Search     Advanced Search   2024 Vol.  21 No.  1 Published: 2024-01-21 Basic Medicine Clinical Medicine Preventive Medicine Nursing Science Basic Medicine 1 ZHANG Gang, GAO Tieming, WU Yang, ZHAO Zhi, ZENG Zhixiang, LI Zheli Effects of IKBKB gene knockdown on NLRC5 and NF-κB expression, as well as the biological function of gastric cancer cells Objective To investigate the relationship between the expression level of the IKBKB gene in gastric cancer cells and the activitiesy of NLRC5 and NF-κB. The effect of IKBKB knockdown on the biological function of gastric cancer cells was clarified. Methods The human gastric cancer SGC-7901 cell line was transfected directly with the plenti-ikkbkb-sgrna gene knockdownout plasmid. Western blot (WB) was used to detect the effect of Ikkbkb gene knockout and the expression levels of NLRC5 and NF-κB in the monoclonal cell line. Scratch test and Transwell migration assay were used to assess the migratory capacity of monoclonal cells, while flow cytometry was employed to measure apoptosis and cell cycle progression. The effect of monoclonal cells on tumor growth was observed in a tumorigenesis experiment. Immunohistochemical staining was used to evaluate the expression status of NLRC5, NF-κB, and IKBKB in clinical gastric cancer tissues and adjacent tissues. To clarify their existence, expression correlation, and clinical diagnostic value. Results After knocking out the IKBKB gene, the expression levels of NLRC5 and NF-κB in human gastric cancer SGC-7901 cells were significantly reduced, and the cell migration and invasion abilities were weakened. G1 phase arrest decreased the proliferation ability of SGC-79013 cells, and the total apoptosis rate of SGC-79013 cells significantly increased. The expression levels of NLRC5 and NF-κB were downregulated after IKBKB knockout, which impacted the growth and development of tumors. The expression levels of NLRC5, NF-κB, and IKBKB in gastric cancer tissues were higher than those in adjacent tissues. Additionally, the expression levels of NLRC5 and NF-κB were positively correlated with IKBKB. The binary logistic regression model was constructed with IKBKB, NLRC5, and NF-κB as explanatory variables. The diagnostic performance of the joint model was quantified using ROC analysis, which showed an AUC of 0.921 with a 95% confidence interval of 0.876-0.967. Conclusion IKBKB has a correlation with NLRC5 and NF-κB in gastric cancer cells, and synergistically promotes the occurrence and development of gastric cancer. 2024 Vol. 21 (1): 1-8 [Abstract] ( 277 ) [HTML 1KB] [PDF 4418KB] ( 1789 ) 8 WEI Yunfu, ZHOU Can, LI Rui, LUO Yutao, HUANG Zhong'en, REN Xiang, SUN Guoying The role of TREM-1/NF- κB pathway in regulation of metformin on mouse acute lung injury Objective This study was designed to examine the effect of metformin on triggering receptors expressed on myeloid cells-1（TREM-1）in a lipopolysaccharide（LPS）induced acute lung injury（ALI）mouse model and explore whether metformin utilizes TREM-1/NF- κB pathway to reduce ALI in mice. Methods First of all,an ALI mouse model was established by intraperitoneal injection of 10 mg/kg LPS,and intervention with metformin or TREM-1 receptor antagonist was used to examine the effect of metformin on TREM-1 expression in ALI mice. Total protein level,total cell and neutrophil count,lactate dehydrogenase（LDH）and myeloperoxidase（MPO）were determined in the lavage fluid（BALF）,and the wet/dry specific gravity of lung tissues were assessed. NF-κB and TREM-1 mRNA expression was assessed through Real time-PCR,while NF-κB,IL-18,IL-1β,TNF-α and TREM-1 protein expression levels were measured by Western blots. Results Our results showed that metformin reduced the expression of TREM-1 in the lung tissue of ALI mice. After antagonizing TREM-1,metformin could alleviate pathological damage,protein exudation,and neutrophil infiltration in lung tissue,and expression of NF-κB,IL-18,IL-1β,TNF-α was inhibited. Conclusion Metformin can reduce the expression of inflammatory factors,vascular permeability,and infiltration of inflammatory cells,thereby alleviating LPS-induced ALI,and its mechanism may be related to TREM-1/NF-κ B pathway. 2024 Vol. 21 (1): 8-14 [Abstract] ( 274 ) [HTML 1KB] [PDF 3456KB] ( 1862 ) 15 LI Haiyang, ZHAO Zhenshan, LI Jing, RONG Yao, ZHENG Aiming, HAO Menghui, TIAN Faming Effect of miR-567 and CacyBP on A549 cells of non-small cell lung cancer Objective To investigate the effects of miR-567 and calcyin-binding protein (CacyBP) on proliferation, invasion and apoptosis of non-small cell lung cancer (NSCLC) A549 cells. Methods NSCLC A549 with high expression of miR-567 and CacyBP was selected as the study object. The experiment was divided into 4 groups, blank control group (NC group), transfected with pcDNA6.2 miR-567(si-miR-567 group). Transfected pcDNA6.2 CacyBP siRNA (si-CacyBP group) and pcDNA6.2 miR-567+CacyBP siRNA group (si-miR-567+CacyBP group); mRNA expressions of miR-567 and CacyBP were detected by qRT-PCR. Cell proliferation was detected by CCK-8 and MTT assay. Transwell assay was used to test cell migration and invasion. The apoptosis rate of NSCLC A549 cells was determined by flow cytometry. The changes of apoptotic proteins in each group were detected by Western bolt. Results Compared with NC group, the expression of miR-567 in si-miR-567 groupand si-miR-567+CacyBP group was significantly decreased. Compared with NC group, the CacyBP mRNA expression in si-CacyBP group and si-miR-567+CacyBP group was significantly decreased. The results of MTT assay showed that the cell proliferation inhibition rate of three groups was significantly higher than that of NC group, and the cell proliferation inhibition rate of si-miR-567+CacyBP group was significantly higher than that of si-miR-567 and si-CacyBP groups. The results of CCK-8 experiment showed that the cell proliferation count of three groups was significantly lower than that of NC group, and the cell proliferation count of si-miR-567+CacyBP group was significantly lower than that of si-miR-567 and si-CacyBP groups. Transwell experiment showed that the number of cell migration and invasion in NC group was significantly higher than that in si-miR-567 group, si-CacyBP group and si-miR-567+CacyBP group. The number of cell migration and invasion in si-miR-567 group and si-CacyBP group was significantly higher than that in si-miR-567+CacyBP group. Flow cytometry showed that compared with NC group, the apoptosis rate of A549 cells in si-miR-567 group, si-CacyBP group and si-miR-567+CacyBP group was significantly increased. The apoptosis rate of A549 cells in si-miR-567+CacyBP group was significantly higher than that in si-miR-567 and si-CacyBP groups. Conclusion Combined intervention of miR-567 and CacyBP can effectively reduce the proliferation, migration and invasion ability of NSCLC A549 cells, and increase the apoptosis rate of cells. 2024 Vol. 21 (1): 15-20 [Abstract] ( 296 ) [HTML 1KB] [PDF 3419KB] ( 1590 ) 20 ZHANG Ying, CHEN Qian, SHI Hua, GUO Jing, XU Jinhong, XU Shuxiong, GONG Maodi Biological effects of Urolithin A on renal carcinoma 786-O cells Objective To investigate the effect of UA on renal carcinoma 786-O cells and the potential mechanism. Methods Pubchem and Swiss target Prediction databases were used to search the structure and target of UA, and the promising targets of UA were picked out according to the probability of action marks, which ware greater than 0. The effects of UA on the viability, migration and invasion of 786-O cells were detected by CCK-8, flow cytometry, Transwell migration and invasion assay, respectively. The expression levels of P62, caspase3, lc3, Bcl-2 and Bax proteins were detected by Western blotting. Results Most of the predicted targets of UA were kinases and cyclins. UA inhibited the proliferation of 786-O cells in a concentration-dependent manner, as well as inhibited the expression of p62 and bcl2 in 786-O cells. And UA inhibited the migration and invasion ability. Moreover, the expression of caspase3, bax and lc3-II were down-regulated in 786-O cells. Conclusion UA can inhibit proliferation, induce apoptosis, inhibit migration and invasion in renal carcinoma cells. The mechanism may be related to inhibiting p62, bcl2 expression and promoting caspase3, bax and lc3-II expression in renal carcinoma cells. 2024 Vol. 21 (1): 20-25 [Abstract] ( 269 ) [HTML 1KB] [PDF 3629KB] ( 2074 ) 26 CHEN Lifang, HUANG Yulang, CHEN Junyu, LUO Xinlin Protective effect of shearstress on LDL-induced endothelial progenitor cell injury through up-regulation of autophagy Objective To analyze the protective mechanism of laminar shear stress (LSS) on endothelial progenitor cells (EPCs) injury induced by oxidized low-density lipoprotein (ox-LDL). Methods The treatment conditions of ox-LDL and LSS in EPCs were analyzed by CCK8. The effects of LSS on the migration and tube formation ability of EPCs before and after treatment with ox-LDL were analyzed by Transwell and tube formation experiments. The effects of LSS and ox-LDL treatment on autophagy and endothelial-mesenchymal transition markers were analyzed by Western blot. The effects of autophagy activators and inhibitors on autophagy and endothelial-mesenchymal transition markers after treatment with ox-LDL and LSS were analyzed by Western blot. Results After treatment with ox-LDL, the proliferation, migration, and tube formation ability of EPCs were down-regulated, and LSS treatment significantly increased them. LSS treatment had a protective effect on ox-LDL-induced EPCs cell injury. In terms of molecular mechanisms, LSS treatment increased the autophagy level of EPCs cells. After treatment with ox-LDL, EPCs cells underwent significant endothelial mesenchymal transition, which was significantly inhibited by LSS treatment. Autophagy activators and inhibitors confirmed these results. Conclusion LSS can inhibit ox-LDL-induced endothelial mesenchymal transformation of EPCs by upregulating autophagy, and plays a protective role in EPCs. 2024 Vol. 21 (1): 26-33 [Abstract] ( 223 ) [HTML 1KB] [PDF 3204KB] ( 1739 ) Clinical Medicine 34 PENG Bo, HUANG Jianliang, XIA Mingkai, HUANG Jiale, LEI Mingsheng Clinical characteristics and treatment analysis of suspected tuberculous pleural effusion Objective Summary of clinical characteristics and treatment of suspected tuberculous pleural effusion (TPE), providing a basis for accurate clinical diagnosis and personalized treatment. Methods This study analyzed clinical data from 24 cases of confirmed TPE and 63 cases of suspected TPE patients at Zhangjiajie People’s Hospital between January 2021 and December 2022. The data included general information, clinical manifestations, auxiliary inspection, treatment measures, and complications. etc. This study aims to compare the TPE group and the suspected TPE group to determine if there are any differences. Results A total of 87 patients were included in this study, with a median age of 49.0(29.0, 65.0) years in the TPE group and 58.0(44.0, 74.0) years in the suspected TPE group. The median age of the suspected TPE group was significantly higher than that of the TPE group, the TPE group exhibited higher levels of lactate dehydrogenase (LDH) at 586.0(346.8, 1016.3) U/L and adenylate dehydrogenase (ADA) at 45.5(31.5, 54.2) IU/L. The median LDH/ADA ratio was 11.6(8.07, 19.4). Yellow turbidity is the most common appearance of pleural effusion, and there is a difference in the appearance of pleural effusion between the TPE group and the suspected TPE group; The most common clinical manifestations included cough, dyspnea, chest pain, fever, and night sweats. All patients tested positive for mucin qualitative analysis; 56(64.4%) patients were positive for tuberculosis antibodies; 85(97.7%) patients with Mycobacterium tuberculosis γ- Positive interferon test, After suspected empirical anti tuberculosis treatment in the TPE group, 61(96.8%) patients had reduced pleural effusion, with 2(3.2%) forming encapsulated effusion. After anti tuberculosis treatment in the TPE group, 23(95.8%) patients had reduced pleural effusion, with 1(4.2%) forming encapsulated effusion. There was no significant difference between the two groups. Conclusion Fully understanding the clinical characteristics of suspected tuberculous pleural effusion, combined with empirical anti tuberculosis treatment, can help improve diagnostic accuracy. 2024 Vol. 21 (1): 34-38 [Abstract] ( 288 ) [HTML 1KB] [PDF 2000KB] ( 1918 ) 39 MO Lihua, DING Jicheng, WO Yeqing, ZHOU Zhihong The Effects of Sufentanil Combined with Ropivacaine for Intrathecal Labor Analgesia on Pain Relief and Postpartum Depression in Women Undergoing Second Childbirth Objective To explore the effects of sufentanil combined with ropivacaine for intrathecal labor analgesia on pain relief and the incidence of postpartum depression in women undergoing their second childbirth. Methods This study included 320 parturients who required analgesia for their second childbirth in our hospital from March 2021 to March 2023. They were randomly divided into a control group and an observation group,with 160 cases in each. The observation group received sufentanil combined with ropivacaine for intrathecal labor analgesia,while the control group did not receive any labor analgesia. The visual analog scale（VAS）scores,Edinburgh Postnatal Depression Scale（EPDS）scores,levels of interleukin 6（IL-6）,tumor necrosis factor（TNF-α）,adrenocorticotropic hormone（ACTH）,cortisol（Cor）,and the incidence of adverse reactions were observed in both groups. Results Compared with the control group,the VAS scores at 6h,12h,and 24h postoperatively were significantly lower in the observation group. There was no significant difference in the preoperative EPDS scores between the two groups;however,the EPDS scores at 3d,7d,and 30d postoperatively were lower in the observation group,as was the incidence of postpartum depression（2.67% vs 8.00%）. The levels of IL-6 and TNF-α preoperatively showed no significant difference between the groups,but were lower in the observation group at 6h,12h,and 24h postoperatively. There was no significant difference in the preoperative levels of ACTH and Cor between the two groups,but the levels were higher at 6h postoperatively in the observation group. The incidence of adverse reactions was not significantly different between the groups. Conclusion Sufentanil combined with ropivacaine for intrathecal labor analgesia in women undergoing second childbirth can improve pain perception,alleviate depressive symptoms,relieve stress,and inhibit inflammatory responses,and is worthy of clinical application. 2024 Vol. 21 (1): 39-42 [Abstract] ( 268 ) [HTML 1KB] [PDF 1985KB] ( 1725 ) 43 ZHANG Chunjiao, HUANG Rong, CAI Tingting, GU Jin Establishment of biological reference interval of renal function test items based on indirect method Objective To establish the reference interval of creatinine, urea and uric acid based on dry chemical system in Jiangsu、Guangzhou and Hebei by indirect method. Methods Collect laboratory information system (LIS) data from Lianyungang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Panyu Maternal and Child Care Service Centre of Guangzhou, and The Affiliated Hospital of Hebei University from January 1, 2023 to June 3, 2023. The K-S test was used to test the normality of the data, the interquartile range method was used to eliminate outliers, the Mann Whitney U test was used to classify the sex and age groups, and the percentage method (P2.5-P97.5) was used to establish the reference interval of each group, and compared with the reference interval of the manual. Results plasma creatinine, urea and uric acid were significantly different between sexes. There is no statistical significance in creatinine between the male under 30 years old group and the 31-60 years old group, and the reference interval can be established in combination. There is statistical significance between the other age groups, and the reference interval needs to be established separately. The biological reference interval of creatinine is 41.59-159.00 umol/L for men over 60 years old, 41.00-138.80 umol/L for men under 60 years old, 35.20-114.27 umol/L for women over 60 years old and 34.20-99.64 umol/L for women over 30 years old under 60 years old. The reference interval of urea is 3.50-11.20 mmol/L for men over 60 years old, 3.10-9.90 mmol/L for men over 30 years old, 2.39-8.95 mmol/L for men under 30 years old, 3.20-10.20 mmol/L for women over 60 years old, and 3.20-10.20mmol/L for women over 30 years old under 60 years old. The reference interval of uric acid is 165.35-570.93 umol/L for men over 60 years old, and 189.13-586.38 umol/L for men over 30 years old under 60 years old. The relative deviation of creatinine in men at all ages, creatinine in women over 60 years old, urea in men over 60 years old and urea in women over 60 years old are greater than RCV, indicating that there is a statistical difference between the self built reference range and the current reference range, and the self built reference range is required. The relative deviation between the self built reference range and the current reference range of other projects is less than RCV, indicating that the difference is not statistically significant. Conclusion There are sex and age differences in creatinine, urea and uric acid . Some items are different from the current reference interval, so it is necessary to establish a reference interval. Indirect method is a feasible method to establish the biological reference range of test items. 2024 Vol. 21 (1): 43-47 [Abstract] ( 261 ) [HTML 1KB] [PDF 2926KB] ( 1608 ) 48 HUANG Wenting, LONG Liang, CHEN Suling, WANG Qiongping, WANG Tao, LONG Fa Clinical effect of pulmonary rehabilitation intervention after bronchial thermoplasty in patients with moderate and severe COPD Objective To explore the clinical efficacy of Delphi pulmonary rehabilitation intervention after bronchial thermoplasty in patients with moderate to severe COPD. Methods 82 patients with moderate to severe COPD in our hospital from January 2021 to January 2023 were selected for study. In the control group, conventional pulmonary rehabilitation was performed after bronchial thermoplasty, and the observation group used Delphi pulmonary rehabilitation on this basis. Compared the clinical efficacy, oxygenation function, quality of life, condition improvement, exercise endurance, number of acute exacerbations, readmission rate and occurrence of respiratory adverse events. Results The apparent efficiency and total efficiency of the patients in the observation group were 82.93% and 95.13% respectively, which were higher than the 63.41% and 85.36% of the control group, and the difference between the two groups was statistically significant when comparing the grades of apparent, effective and ineffective. The difference between the two groups before and after the intervention was statistically significant; The differences of PCO2, respiratory index and oxygenation index before and after intervention in observation group were higher than those in control group. The difference of SGRQ, CAT and 6MWT before and after intervention in the observation group was compared, and the difference was statistically significant after t test; The differences of SGRQ, CAT and 6MWT before and after intervention in the observation group were higher than those in the control group. There was no significant difference in readmission rate between observation group and control group. The incidence of respiratory related adverse events in the observation group was lower than that in the control group 3 months after surgery. Conclusion Delphi pulmonary rehabilitation in patients with moderate to severe COPD can effectively improve clinical efficacy, improve oxygenation function, improve quality of life, improve disease condition, improve exercise endurance, and reduce the number of acute exacerbations and the incidence of complications. 2024 Vol. 21 (1): 48-52 [Abstract] ( 235 ) [HTML 1KB] [PDF 1949KB] ( 1677 ) 52 LI Juan, CAI Hehua, WANG Zhiqiang, CHEN Guihua, WU Xiaoyan, LIU Qianyang Construction and evaluation of nomograph model for individualized prediction of recurrent respiratory tract infection risk in children in chengdu Objective To construct an individualized nomogram model for predicting the risk of recurrent respiratory tract infections in urban children in Chengdu, and to evaluate the accuracy and clinical applicability of this nomogram model. Methods Children with respiratory tract infections admitted to our hospital recently were selected as the study subjects. The children were divided into two groups: those with recurrent respiratory tract infections and those with ordinary respiratory tract infections.70% of the subjects were randomly selected as the training set, and the remaining 30% were used as the testing set. Logistic regression analysis was conducted on the training set to identify independent risk factors for recurrent respiratory tract infections in Chengdu children. Based on the identified risk factors, a nomogram model was constructed. The calibration curve was drawn after performing 1000 rounds of bootstrap sampling on the training set. Internal validation of the nomogram model was performed using ROC curves and clinical decision curves, and the clinical efficacy of the model was validated on the testing set. Results Logistic regression analysis on the training set revealed the following independent risk factors for recurrent respiratory tract infections in urban children in Chengdu: history of allergies, history of asthma, low levels of vitamin A and vitamin D, low birth weight, passive smoking, and low hemoglobin levels. The C-index of the nomogram model in the training set was 0.879(95% CI 0.812-0.932). The calibration curve showed that the predicted risk of recurrent respiratory tract infections in children using the nomogram model was close to the actual observed curve. The clinical decision curve indicated that the nomogram model was most applicable when the predicted risk of recurrent respiratory tract infections in urban children in Chengdu was between 0.04 and 0.95. The ROC curve showed an AUC of 0.921(95% CI 0.851-0.968), sensitivity of 0.848, and specificity of 0.872 for the model. In the testing set, the ROC curve showed an AUC of 0.912(95% CI 0.871-0.941), sensitivity of 0.845, and specificity of 0.836. Conclusion The constructed individualized nomogram model for predicting the risk of recurrent respiratory tract infections in urban children in Chengdu demonstrates good accuracy and clinical applicability. 2024 Vol. 21 (1): 52-57 [Abstract] ( 249 ) [HTML 1KB] [PDF 2341KB] ( 1567 ) 58 KONG Lingguo, HAN Xudong, GE Liang, ZHANG Peng, LI Shengbin, LI Na Effect of dexmedetomidine on epidural labor analgesia through mapk/ERK signaling pathway Objective To investigate the effect of different doses of dexmedetomidine on epidural labor analgesia for mothers and infants based on MAPK/ERK signaling pathway. Methods 200 puerperae who met the inclusion criteria were randomly divided into: non-epidural analgesia group (group C), epidural analgesia combined with normal saline group (group N), epidural analgesia combined with intravenous injection of dexamethasone Detomidine 0.5μg/kg group (group D1), epidural analgesia combined with intravenous injection of dexmedetomidine 0.75μg/kg group (D2 group) and epidural analgesia combined with intravenous injection of dexmedetomidine 1μg/kg group (D3 group), 40 cases in each group. The Ramsay sedation scale and VAS score were used to record the pain scores of sedatives in each group. The volume method was used to detect the amount of vaginal bleeding at 2 h postpartum. RT-PCR was used to detect the mRNA and protein expressions of Raf, ERK, CREB and DYN in the placenta. A rat pregnancy model was established, and the successfully modeled pregnant rats were randomly divided into 4 groups, namely blank control group C, dexmedetomidine 50 μg/kg (group D1), dexmedetomidine 100 μg/kg (group D2) ), dexmedetomidine 200 μg/kg (group D3). The pain threshold of rats during childbirth was determined by hot water tail flick method, and the Richmond agitation and sedation scale was used for scoring; RT-PCR and WB were used to detect the parietal lobe region of gray matter, spinal cord lumbar enlargement, and myometrium tissue Raf, ERK, Changes in mRNA and protein content of CREB and DYN. Results TResults There were no significant differences in age, BMI, gestational week, cervical dilation, postpartum vaginal bleeding volume, pre-analgesic Ramsay score, pre-analgesic VAS score and neonatal Apgar score among all groups. The Ramsay score in T1-T3 in D1-D3 group was higher than that in N group, the VAS score was lower than that in N group, the mRNA of Raf, ERK and CREB in D1-D3 group was significantly lower than that in N and C group, and the mRNA was significantly higher than that in N and C group, with statistical significance. In the rat pregnancy model, compared with group C, administration of 100μg/kg and 200 μg/kg dexmedetomidine increased tail flick latency, Richmond agitation sedation score was lower than group C. Dexmedetomidine group decreased the mRNA and protein contents of MAPK/ERK signaling pathway in the parietal region of brain gray matter, lumbar enlargement of spinal cord and myometrium of rats, and the differences were statistically significant. Conclusion Dexmedetomidine is used for epidural labor analgesia. By reducing the expression of MAPK/ERK signaling pathway, it can effectively relieve labor pain without obvious maternal. 2024 Vol. 21 (1): 58-65 [Abstract] ( 276 ) [HTML 1KB] [PDF 2917KB] ( 1720 ) 66 SU Qiyan, HUANG Biao, HU Aili, PAN Hong, HU Chongyu Correlation analysis of risk factors for white matter hyperintensities in cerebral small vessel disease Objective To evaluate the risk factors of white matter hyperintensities (WMHs) in Cerebral small vessel disease (CSVD) , and to further establish an early prediction model. Method A total of 233 patients hospitalized in Hunan Provincial People's Hospital were selected for retrospective analysis. According to the T2 sequence of head magnetic resonance plain scan to identify WMHs, they were divided into the observation group of 187 cases with WMHs and the control group of 46 cases without WMHs. The general data and related biochemical indicators of the two groups of patients in the training set were compared between the two groups, and the difference factors were analyzed by multivariate Logistic regression analysis. The receiver operating characteristic (ROC) curve was drawn for single and multiple independent risk factors, and a nomogram was constructed to establish a prediction model, and the predictive efficacy of the model was evaluated. Results There were significant differences in age, hypertension, coronary heart disease, monocyte count, blood urea nitrogen (BUN) , serum creatinine (SCr) , and glomerular filtration rate (eGFR) between the two groups of patients in the training set (P<0.05) . Multivariate regression analysis of 7 indicators showed that hypertension and SCr level were independent risk factors for WMHs. The area under the ROC curve of hypertension, SCr and the two indexes combined to predict WMHs was 0.655, 0.680 and 0.762, respectively. By constructing a nomogram for hypertension and SCr, the efficacy of the prediction model and the ROC curve of WMHs in the hypertension and SCr diagnostic validation set alone was evaluated, and the curve area was 0.763, 0.708, and 0.649, respectively. Conclusions Hypertension and SCr are independent risk factors for WMHs, and have predictive value for WMHs. The combination of hypertension and SCr is better than single index in predicting the risk of WMHs, which has guiding value for the early diagnosis of WMHs. 2024 Vol. 21 (1): 66-71 [Abstract] ( 263 ) [HTML 1KB] [PDF 2572KB] ( 1646 ) 71 LI Wenjun, LIU Wei, LIN Zhuohui, ZHU Liya Effect of Bushen Huoxue Jianpi Decoction in the treatment of polycystic ovary syndrome complicated with osteopenia and the impact on reproductive hormones,ovulation and bone metabolism Objective To observe the effect of treatment with Bushen Huoxue Jianpi Decoction on serum reproductive hormones,ovulation and bone metabolism in patients with PCOS and osteopenia. Methods A total of 106 patients with PCOS and osteopenia who were treated in the First Clinical Medical College,Guangzhou University of Traditional Chinese Medicine from May 2021 to April 2023,and met the inclusion and exclusion criteria were enrolled in this study. They were randomly assigned to the treatment group and the control group,with 53 patients in each group. Patients in the control group were treated with Diane-35 combined with olive oil. On this basis,patients in the treatment group were treated with Bushen Huoxue Jianpi Decoction. Reproductive hormones（FSH,LH and E2）,ovulation,menstruation and bone metabolism（BGP and PINP）in the two groups were observed. Clinical efficacy was evaluated. Results After treatment,ovarian volume,endometrial thickness,follicle count,duration and volume of menstruation,serum BGP and PINP in the two groups were significantly increased. Menstrual cycle and serum FSH,LH and E2 were significantly reduced. Meanwhile,ovarian volume,endometrial thickness,follicle count,duration and volume of menstruation,serum BGP and PINP in the treatment group were larger/longer/higher than those in the control group（P<0.05）. Menstrual cycle and serum FSH,LH and E2 in the treatment group were shorter/lower than those in the control group（P<0.05）. The total effective rate in the treatment group was significantly higher. Conclusion Treatment with Bushen Huoxue Jianpi Decoction can not only improve reproductive hormone levels and effectively correct ovulation and menstruation in patients with PCOS and osteopenia but also positively regulate bone metabolism,with significant effect. 2024 Vol. 21 (1): 71-75 [Abstract] ( 256 ) [HTML 1KB] [PDF 1924KB] ( 1828 ) 75 LI Xiaogui, WUYi, LU Jiayin, LI Hao, WANG Xintian Clinical study on curative effect of surgical treatment for hepatolithiasis Objective To investigate the surgical treatment of hepatolithiasis. Methods Retrospective analysis and summary were made of 658 cases of hepatolithiasis treated by our department of surgery from January 2019 to July 2022, and the characteristics of the cases and surgical methods were analyzed . Results Among the 658 cases, 306 cases (46.50%) underwent hepatic lobectomy, among which laparoscopic hepatic lobectomy accounted for 13.7%; biliary and intestinal drainage accounted for 52.58%; holmium laser accounted for 46.20%; percutaneous hepatic bile duct lithotripsy (percutaneous transhepatic choledochoscopic lithotripsy (PTCSL) accounted for 6.69%. There was 1 death in the whole group, and 196 patients (29.79%) were found to have residual stones in postoperative CT scans, of which 63 patients (9.57%) underwent choledochoscopy for stone removal.19cases (2.89%) of complicated biliary tract infection occurred after operation, of which 2 case (0.31%) died after multiple organ failure due to severe infection, and the remaining 17 cases ( (2.58%) were treated with repeated bile culture to adjust antibiotics and other symptomatic cases improved after supportive treatment. There were 52 cases (7.90%) of postoperative bile leakage, all of which were self-limited after nutritional support therapy or ultrasound-guided intraperitoneal drainage. There were 50 cases (7.60%) of postoperative hemorrhage, of which 4 cases (0.61%) underwent laparotomy for hemostasis, 3 cases (0.46%) underwent interventional embolization for hemostasis, and the rest stopped bleeding after conservative treatment. Conclusion The disease of hepatolithiasis is complicated and personalized, which requires multi-departmental collaboration and combination of a variety of advanced technologies to formulate “individual” treatment plan for specific cases. 2024 Vol. 21 (1): 75-80 [Abstract] ( 275 ) [HTML 1KB] [PDF 2597KB] ( 1794 ) 81 LI Wenhui, FANG Cheng, YAN Fengshang, XU Mingjuan Influence of laparoscopic nerve-sparing radical hysterectomy on postoperative bladder function, sexual function, serum leptin and Hcy in cervical cancer patients Objective To investigate the influence of laparoscopic nerve-sparing radical hysterectomy（LNSRH）on postoperative bladder function,sexual function,serum leptin and homocysteine（Hcy）levels in patients with cervical cancer. Methods The clinical data of 220 cervical cancer patients who were admitted to the hospital between November 2020 and November 2022 were collected retrospectively. According to different surgical methods,the patients were divided into the control group（n=110）and observation group（n=110）. The control group was treated with laparoscopic radical hysterectomy（LRH）,while the observation group was treated with LNSRH. Surgical conditions,postoperative recovery,postoperative bladder function,sexual function,serum leptin and Hcy levels,and postoperative survival of the two groups were comparatively analyzed. Results Surgical time of the observation group was longer than that of the control group,and postoperative hospital stay was shorter than that of the control group . There was no significant difference between the two groups in terms of intraoperative blood loss,the length of vagina removed,the incidence of urinary incontinence,and the incidence of urinary retention. The time of catheter removal,and the incidence rates of bladder dysfunction and urination with abdominal pressure in the observation group were significantly better than those in the control group. After operation,serum leptin and Hcy levels in the two groups were lower than those before surgery. Meanwhile,serum leptin and Hcy levels in the observation group were lower than those in the control group. After operation,the scores for sexual desire,sexual arousal and orgasm,vaginal lubrication and dyspareunia,and satisfaction with sexual life in the observation group were higher than those in the control group. No death case was found in either group. The recurrence rates in the observation group and the control group were 2.73% and 6.36%,without significant difference. Conclusion LNSRH can promote postoperative recovery of cervical cancer patients,improve their postoperative bladder function and sexual function,and effectively reduce serum leptin and Hcy levels,with good safety. 2024 Vol. 21 (1): 81-84 [Abstract] ( 236 ) [HTML 1KB] [PDF 1957KB] ( 1897 ) 85 LIANG Yu, LIU Huanhao, MAI Haiyan, HUANG Yu, LIANG Jinyue Effect of Early Urokinase Sealing on Orevention and Treatment of Catheter Fibrin Sheath Formation in Long-term Hemodialysis Patients Objective To observe the effect of early urokinase sealing in preventing the formation of catheter fibrin sheath in long-term hemodialysis patients. Methods From February 2021 to February 2022,46 patients were selected for regular hemodialysis treatment in the hemodialysis room and nephrology department of our hospital. The patients were divided into two groups. In the urokinase group,23 patients began to receive urokinase tube sealing treatment at the time of the first dialysis treatment,while in the conventional group,23 patients only received conventional heparin tube sealing treatment at the time of the first dialysis treatment,and urokinase therapy was started only after the patient's catheter was dysfunctional. The occurrence of catheter dysfunction,the change of coagulation function index before and after treatment,the change of catheter blood flow and venous pressure during treatment and the occurrence of adverse reactions were compared between the two groups. Results The occurrence of catheter dysfunction in the urokinase group was much lower than that in the conventional group,and the average time of the first occurrence of catheter dysfunction in the urokinase group was later than that in the conventional group. At 6 months of dialysis,there was no significant difference in APTT,TT and Fib levels between the two groups,but the average PT time of the urokinase group was significantly longer than that of the conventional group. There was no significant difference in the mean catheter blood flow and venous pressure between the two groups at 1 month of dialysis. At 3 and 6 months of dialysis,the average blood flow in the urokinase group was significantly higher than that in the conventional group,while the venous pressure was significantly lower than that in the conventional group. Neither urokinase group nor routine group had any serious adverse reactions that needed to stop treatment,no gastrointestinal bleeding,serious liver injury and other drug toxicity. Conclusion In patients who need long-term catheterization for hemodialysis treatment,early urokinase sealing measures can better prevent the formation of fibrin sheath in the catheter,prolong the service life of the catheter,and have high safety. 2024 Vol. 21 (1): 85-89 [Abstract] ( 291 ) [HTML 1KB] [PDF 2190KB] ( 1409 ) 90 LIU Quan, WEI Hongxia Research on the effect of Ilex pubescens extract on wound repair of mice with radiation oral mucositis model Objective To investigate the effect of Ilex pubescens extract on the wound healing effect of mice with radiation oral mucositis and to explore its possible mechanism. Methods Male C3H mice with 8 weeks old were selected to reproduce the model of radiation oral mucositis（RTOM）in mice by X-ray irradiation of the oral and tongue parts. They were randomly divided into model group,positive control and experimental groups. HE staining was used to observe the recovery of mucosal lesions and the density of fibroblasts. The expression of IL-6 and TNF-α protein was detected by immunohistochemistry. Results The mouse RTOM model was successfully replicated. After 7 days,the model group mice showed grade III oral ulcer,thinning of the oral mucosal epithelial layer,slight disorder of keratinized epithelial structure,dilation of capillaries,and visible infiltration of inflammatory cells;The mice in the cell growth factor group exhibited grade II oral ulcer,slightly increased thickness of buccal mucosal epithelium compared to the normal group,and normal keratinized epithelial structure;After 7 days of treatment,the mice in the Ilex exract group showed grade I oral ulcer,increased keratinization of the buccal mucosa,and normal keratinized epithelial structure. Immunohistochemical and Western blot results showed that the expression of TNF - and IL-6 in the cheek mucosa of mice in the cell growth factor group and Ilex exract group was significantly lower than that in the model group. The expression of TNF - and IL-6 in the cheek mucosa of mice in the Ilex exract group was significantly lower than that in the cell growth factor group. The positive rates of TNF - and IL-6 expression in the cell growth factor group and Ilex exract group were significantly lower than those in the model group. The positive rates of TNF - and IL-6 expression in the Ilex exract group were significantly lower than those in the cell growth factor group. Conclusion Extracts of Ilex have a repairing effect on ulcer wounds in mice with RTOM. 2024 Vol. 21 (1): 90-95 [Abstract] ( 260 ) [HTML 1KB] [PDF 2655KB] ( 2021 ) 95 LONG Lei, LI Qing, YI Zhuozhuo Effects of blood glucose fluctuation on the prognosis of patients with type 2 diabetes mellitus and sepsis Objective To investigate the impact of blood glucose fluctuations on the prognosis of patients with type 2 diabetes mellitus (T2DM) and sepsis. Methods A total of 125 patients with T2DM and sepsis who were admitted to a tertiary hospital in Hunan Province from March 2021 to March 2023 were selected. Based on the 28-day outcome, the patients were divided into two groups: the non-survival group (n=31) and the survival group (n=94). fasting blood glucose (FBG), postprandial blood glucose at 2 hours (2hPG), glycated hemoglobin (HbA1c), mean blood glucose, blood glucose standard deviation (SDBG), postprandial blood glucose fluctuation amplitude (PPGE), maximum blood glucose fluctuation amplitude (LAGE), blood glucose coefficient of variation (CV), time in range (TIR), acute physiology and chronic health evaluation II (APACHE II) score, and other indicators were collected. The differences between the two groups were compared for statistical significance. Spearman correlation analysis was performed between mean blood glucose, SDBG, PPGE, LAGE, CV, TIR and APACHE II score. Results The FBG, 2hPG, and HbA1c levels were no significant difference between the two groups; Compared with the survival group, the non-survival group had higher APACHE II scores, mean blood glucose, SDBG, PPGE, LAGE, and CV, but lower TIR. There were significant differences between the two groups; Mean blood glucose, SDBG, and LAGE were positively correlated with APACHE II scores, while TIR was negatively correlated with APACHE II scores. There was statistical significance. CV and PPGE were not significantly correlated with APACHE II scores. Conclusion Blood glucose fluctuations are associated with the prognosis of patients with diabetes mellitus and sepsis, increasing the risk of adverse outcomes. 2024 Vol. 21 (1): 95-98 [Abstract] ( 304 ) [HTML 1KB] [PDF 1966KB] ( 1834 ) 98 HAO Yongjie, DENG Junji, FENG Kun, DU Hanwang, CHEN Wei, WEI Yao, CUI Chuanshui Application of magnetic resonance apparent diffusion coefficient imaging in differentiating lung cancer from benign lung lesions Objective To evaluate the application effect of different MRI diffusion-weighted imaging models based on the apparent diffusion coefficient in distinguishing lung cancer from benign lung lesions. Methods This retrospective single-center study selected 49 patients from January 2020 to June 2021 as the study subjects, including the lung cancer group (n=32) and the benign disease group (n=17). All patients underwent diffusion-weighted imaging (DWI) before surgery or biopsy. ADC histogram parameters, including mean, percentile values (10th and 90th), kurtosis, and skewness, were calculated independently by two radiologists. The histogram parameters were compared between patients with lung cancer and benign lesions. ROC curves were constructed to evaluate the diagnostic performance. Results The ADCmean, ADC10th, Dmean and D10th in the lung cancer group were significantly lower than the benign lesion group, and the ADCskewness and Dskewness were significantly higher than the benign lesion group. D10th and ADC10th had the best ability to diagnose benign lesions. The area under the curve (AUC) of D10th was 0.851, and the accuracy was 79.6% at the best critical value of 0.453×10-3 mm/s; the AUC of ADC10th was 0.814, and the accuracy was 83.4% at the best critical value of 0.504×10-3 mm/s. Conclusions Whole-lesion histogram analysis of ADC parameters derived from the DWI single-index and double-index models can distinguish lung cancer from benign lesions. 2024 Vol. 21 (1): 98-102 [Abstract] ( 248 ) [HTML 1KB] [PDF 3447KB] ( 1772 ) 103 LIU Peihui, ZHAO Jinyan, JIANG Yang, CAO Xiaopan, BA Yi, CHEN Ying, SHEN Di, SHEN Daguang Construction and evaluation of nomogram model for short-term prognosis of elderly patients with acute cerebral infarction treated with mechanical thrombectomy Objective To construct a nomogram model for the short-term prognosis of elderly patients with acute cerebral infarction treated with mechanical thrombectomy, and to evaluate the accuracy and clinical applicability of the model. Methods The clinical data of elderly patients with acute cerebral infarction who underwent mechanical thrombectomy in three high-end stroke centers were retrospectively analyzed. They were divided into good prognosis group and poor prognosis group according to the mRS score after 90 days. Logistics analysis was used to analyze the causes of acute cerebral infarction in the elderly. Independent risk factors for patients’ short-term poor prognosis after mechanical thrombectomy. Based on the above independent risk factors, a nomogram model was constructed using R language. The ROC curve, Bootstrap method and clinical decision curve were used to verify the accuracy of the model and the benefits of clinical decision-making. Results The independent risk factors for poor short-term prognosis in elderly patients with acute cerebral infarction after mechanical thrombectomy are: NIHSS score on admission, diabetes mellitus, femoral artery puncture to recanalization time (PTR), postoperative pulmonary infection, postoperative complications Later, symptomatic cerebral hemorrhage occurred and collateral circulation was poor. The ROC curve validation nomogram model shows that the constructed nomogram model has strong predictive ability to predict the short-term prognosis of elderly patients with acute cerebral infarction treated with mechanical thrombectomy (AUC=0.876, 95%CI 0.71-0.94). The Bootstrap method shows the calibration curve. The average absolute error is 0.015, indicating that the calibration curve fits well with the ideal curve. The clinical decision curve shows that the nomogram model has the best applicability when the incidence threshold for predicting the short-term prognosis of elderly patients with acute cerebral infarction after mechanical thrombectomy is between 0.04 and 0.95. Conclusion The constructed nomogram model has high prediction accuracy and has wide clinical applicability. 2024 Vol. 21 (1): 103-110 [Abstract] ( 264 ) [HTML 1KB] [PDF 2326KB] ( 1559 ) 110 SONG Tienan, ZHAO Deyuan, TONG Yongjian, SONG Bo, XU Gang Effect of metatarsophalangeal joint fusion combined with metatarsophalangeal arthroplasty in the treatment of rheumatoid forefoot deformity in elderly patients Objective To explore the short-term efficacy of metatarsophalangeal joint fusion combined with metatarsophalangeal arthroplasty for rheumatoid forefoot deformity in the elderly. Methods Ninety-two elderly patients with rheumatoid forefoot deformity admitted to our hospital from July 2019 to July 2022 were selected as the subjects of this study, and all of them were treated with metatarsophalangeal joint fusion. The patients were followed up for two months to observe the changes of the hallux valgus Angle (HVA) and the 1st to 2nd intermetatarsal Angle (IMA) before and after surgery. American Society for Foot and Ankle Surgery (AOFAS) score, plantar pressure, pain scale (VAS), Berg Balance Scale (BBS) score, Modified Barthel Index (MBI) score and quality of life score of patients were recorded before and after surgery. Results Compared with preoperative results, postoperative HVA and IMA were decreased, pain, function, toe line of force and total score of AOFAS were increased, pressure in all parts of plantar was decreased, VAS score was decreased, BBS score and MBI score were increased. The scores of all dimensions of quality of life were higher than those before operation . During the follow-up period, among the 92 patients, there was 1 case of bone nonunion of the first metatarsophalangeal joint after surgery, 3 cases of delayed incision healing, and no wound infection, toe necrosis, recurrence, or plantar callose formation. Conclusion Metatarsophalangeal joint fusion combined with metatarsophalangeal arthroplasty has a significant short-term effect in elderly patients with rheumatoid forefoot deformity, which can reduce HVA and IMA, improve pain, reduce plantar pressure, improve quality of life, and effectively improve the structure and dysfunction of elderly patients with rheumatoid forefoot deformity. 2024 Vol. 21 (1): 110-114 [Abstract] ( 310 ) [HTML 1KB] [PDF 1965KB] ( 1860 ) 115 WU Xianlin, YIN Zhuan, FANG Yuqi, XIANG Qiuhong, YANG Xiaoqin, XIONG Lijuan Retrospective Analysis of Factors Influencing PICC-Related Bloodstream Infections in NICU Low Birth Weight Infant Objective To explore the influencing factors of peripherally inserted central catheter (PICC) -related infections in low birth weight infants in the Neonatal Intensive Care Unit (NICU) and provide a basis for PICC placement in NICUs. Methods We included low birth weight infants who were hospitalized in a tertiary hospital in Changsha City from January 2021 to June 2022 and underwent PICC placement. We retrospectively analyzed their clinical data, used a case-control method, with those who experienced catheter-related infections as the case group and those who did not as the control group. We conducted univariate and multivariate logistic regression analyses to investigate the risk factors for catheter-related bloodstream infections. Results A total of 19 low birth weight infants developed catheter-related bloodstream infections during a total catheterization duration of 3,466 days, with an infection rate of 0.5% per catheter day. The results of logistic regression analysis showed that birth weight<1 000 g (OR=0.266, 95% CI 0.084-0.842) and catheterization age≤7 days (OR=0.193, 95% CI 0.057-0.653) were risk factors. Birth weight and the age at catheter placement, when combined, have a maximum area under the curve of 0.741 and an approximate odds ratio of 0.369. This combination is more effective in predicting Catheter-related bloodstream infection in low birth weight infants compared to individual factors alone. Conclusion For low birth weight infants, especially those with a birth weight of less than 1 000 g and premature infants with catheter placement at ≤7 days of age, increased vigilance and preventive measures should be taken to reduce the occurrence of Catheter-related bloodstream infection. 2024 Vol. 21 (1): 115-119 [Abstract] ( 255 ) [HTML 1KB] [PDF 1953KB] ( 1862 ) 119 LIU Jianxin, WANG Gang, LEI Guangwen The expression of S-100B, LP-PLA2 and circ_0021132 in acute cerebral infarction and their relationship with recurrence Objective To explore the expression of acid calcium binding protein (S-100B), lipoprotein-associated phospholipase A2(LP-PLA2), and circular RNA (circ_0021132) in acute cerebral infarction (ACI) and their relationship with recurrence. Methods From January 2019 to October 2020, 138 patients with ACI in our hospital were selected as the observation group, and 99 patients with healthy physical examination during the same period were selected as the control group. The blood levels of S-100B, LP-PLA2, and circ_0021132 were compared between the two groups. The observation group was followed up for 1 year to record the ACI recurrence rate. Cox regression method was used to analyze the related factors of ACI recurrence. The receiver operating characteristic curve (ROC) was used to analyze the predictive value of S-100B, LP-PLA2, circ_0021132 in recurrence. Results Serum S-100B [(1.21±0.20) μg/L vs (1.53±0.25) μg/L] and LP-PLA2 [(180.26±36.49) μg/L vs (238.72±49.75) μg/L] levels in the observation group decreased after treatment, and circ_0021132 [(1.61±0.18) vs (1.32±0.09)] levels increased. Serum S-100B and LP-PLA2 levels before and after treatment were higher than those in the control group, and circ_0021132 levels were lower than those in the control group (P<0.05). The expression of circ_0021132 increased after treatment of different types of cerebral infarction (P<0.05). circ_0021132 of patients with large atherosclerosis, cardiogenic embolization and small artery occlusion were lower than those with other clear and unknown etiology (P<0.05). After adjusting for cerebral infarct volume, smoking, carotid plaque, diabetes mellitus and hyperlipidemia by multivariate Cox regression equation, anti-platelet aggregation drugs, serum S-100B, LP-PLA2, and circ_0021132 levels after treatment remained were independent influencing factors of recurrence (P<0.05). The AUC of S-100B, LP-PLA2, and circ_0021132 combined to predict the recurrence of ACI was 0.897, and the PI value corresponding to the maximum Youden index was 0.17. With the increase of PI value (<0.1, 0.1~0.2, >0.2), the recurrence rate (0 vs 9.84% vs 32.69%) showed an increasing trend. Conclusion S-100B, LP-PLA2, and circ_0021132 expressed abnormally in ACI patients, and they are independent factors of ACI recurrence. Combined detection can provide an objective basis for clinical assessment of the risk of ACI recurrence. 2024 Vol. 21 (1): 119-124 [Abstract] ( 271 ) [HTML 1KB] [PDF 2047KB] ( 2004 ) 124 DUAN Guangmin, ZHAO Jing, FU Xiaomei The predictive value of blood gas parameters on curative effect and prognosis of neonatal pneumonia treated with high-flow nasal catheter Objective To investigate the effect and predictors of failure of high-flow nasal cannula (HFNC) as the primary respiratory support therapy for non-invasive ventilation in severe neonatal pneumonia (NSP). Methods This study retrospectively analyzed 85 neonates with NSP admitted to the neonatal intensive care unit of our hospital from January 2019 to December 2021. All patients received HFNC as primary supportive care. HFNC failure was defined as the need for escalation to noninvasive ventilation or invasive mechanical ventilation. Based on clinical data obtained before HFNC treatment and at 30, 60, 120, and 720 min after treatment, HFNC success (responders) and HFNC failure groups (non-responders) were compared. Results Among 85 neonatal infants, 13 patients (15.29%, 13/85) experienced HFNC failure, and 72 patients (84.71%, 72/85) were successfully ventilated. Compared with the failed HFNC group, the gestational age, birth weight, and duration of HFNC in the successful HFNC group were significantly increased, as well as the respiratory distress score and the length of hospital stay were significantly decreased. The comparison of baseline gas and blood parameters showed that the RS of the successful HFNC group was significantly lower than that of the failed HFNC group, and the SpO2/FiO2 (S/F), SpO2 and pH were significantly increased. Gestational age, severity of respiratory distress, RS and S/F predicted HFNC failure according to logistic regression analysis. After HFNC treatment, RR, HR, RS and FiO2 were significantly decreased in both groups, and SpO2 and S/F ratio were increased. Compared with the successful HFNC group, the HFNC failure group had significantly lower RS and FiO2 after HFNC treatment, and increased SpO2 and S/F ratio. The achievement rate of S/F goals at 30 min and 60 minutes, RR goals at 30 minutes, and RS goals at 30 minutes was higher in the HFNC success group compared with the HFNC failure group. In logistic regression analysis, the 60-min S/F target significantly predicted successful HFNC treatment (OR=8.035, 95%CI 2.980-21.658). Conclusions HFNC is suitable for the treatment of respiratory distress/failure of NSP newborns, and S/F ratio can be used as an early warning index to monitor the failure of treatment before or 60 minutes after HFNC treatment. 2024 Vol. 21 (1): 124-128 [Abstract] ( 275 ) [HTML 1KB] [PDF 2138KB] ( 1497 ) Preventive Medicine 129 GAO Xiong, SUN Chang, FANG Yaqing, GAO Han, WANG Yefei, CAO Rui The relationship between time-related personality traits and sleep fatigue, a mediating role for bedtime procrastination Objective Based on the theory of time motivation, this study aimed to explore the mediating role of time-related personality traits (future focus and impulsivity) on fatigue and sleep procrastination. Methods 437 undergraduate students from a university in Changsha were selected using random whole-cluster sampling, and questionnaires were administered using the Time Focus Scale (TFS), the Brief Barratt Impulsivity Scale (BBIS), the Sleep Procrastination Scale (BPS), and the Fatigue Scale (FS-14). Results The undergraduate students' future focus score was (19.16±4.07), impulsivity score was (18.30±3.66), sleep procrastination score was (29.98±6.37), and fatigue score was (8.03±3.23); regression analyses showed that future focus negatively predicted bedtime procrastination (β=-0.281, P<0.01), impulsivity positively predicted bedtime procrastination (β=0.297, P<0.01), future focus negatively predicted fatigue (β=-0.017, P<0.01), and impulsivity positively predicted fatigue in a positive correlation (β=0.664, P<0.01); future focus and impulsivity not only directly predicted college students' fatigue symptoms, but also indirectly predicted college students' fatigue symptoms through bedtime procrastination, the direct effects were 0.514 and 0.0586, and the indirect effects were 0.150 and 0.0942, respectively. Conclusion Future focus and impulsivity can not only directly affect college students' fatigue symptoms, but also indirectly predict college students' fatigue symptoms through sleep procrastination. School educators can take effective measures to improve the phenomenon of bedtime procrastination and fatigue among college students by combining relevant factors, so as to improve students' learning status. 2024 Vol. 21 (1): 129-134 [Abstract] ( 272 ) [HTML 1KB] [PDF 2124KB] ( 1821 ) 135 LIN Leshi, LI Wenjuan, TENG Sheng, Yang Yi, LI Li, PENG Fang, PENG Dongmei, QIN Lullu, GAO Xiao Serum Levels of Per- and polyfluoroalkyl Substances and Their Determinants in Early Pregnant Women in Changsha City Objective To explore the level of per- and polyfluoroalkyl substances (PFAS) in Changsha’s early pregnant women and the influencing factors of per-and polyfluoroalkyl substances, providing the prenatal care and evidence of concentration of PFAS in pregnant women. Method From among pregnant women who underwent prenatal examinations at Changsha Maternal and Child Health Hospital in Hunan Province in 2022, we selected participants for a questionnaire survey and blood sample testing. We collected pregnancy-related information, performed a descriptive analysis of PFAS concentration, and conducted a multiple linear regression analysis to identify factors that affect PFAS concentration. Results 23 PFAS were detected in the serum of pregnant women in Changsha, among which 10 compounds had a detection rate of 100%, and 13 compounds had a detection rate exceeding 90%. The median concentration of total PSASs was 7.572 9 ng/mL. The median concentrations of perfluorooctane sulfonic acid (PFOS) and perfluoro-n-octanoic acid (PFOA) were 2.369 6 ng/mL and 1.773 8 ng/mL. The multiple linear regression results showed that smoking, age, residence cleaning frequency, drinking and gravidity were the influencing factors of PFAS concentration. Conclusion The concentration of PFAS of pregnant women in Changsha is similar to that of other inland cities. The positive correlation factors affecting the level of PFAS are smoking, age and residence cleaning frequency; the negative correlation factor is drinking and gravidity. 2024 Vol. 21 (1): 135-143 [Abstract] ( 246 ) [HTML 1KB] [PDF 2559KB] ( 1841 ) 143 JI Qiaoxue, ZHAO Jie, BU Feifei, WANG Chuanbo Epidemiological analysis of outpatients in Juba Teaching Hospital, South Sudan Objective To study and understand the epidemiological characteristics of outpatients in physiotherapy department of Juba teaching Hospital. Methods The general information of 3 369 outpatients who visited the physiotherapy department of Juba Teaching Hospital from March 2, 2021 to November 17, 2022 was collected. The ECXEL database was established, and the statistical software R was input to retrospectively describe and analyze the regular characteristics of the patients, such as gender, age, reason for seeking medical treatment, time of seeking medical treatment, treatment methods and the correlation among various factors. Results Spine disease was the main diseases, accounting for more than 50%. Male patients in local physiotherapy department were significantly higher than female patients, and male patients with various diseases were higher than female patients. The patients were mainly young and middle-aged. The number of outpatient visits in rainy season was significantly higher than that in dry season. The treatment method is simple, single and backward. Conclusion In the future foreign medical aid work, more treatment methods adapted to local conditions can be added according to the characteristics of patients in the local physiotherapy department, and full preparation of internal and external use of proprietary Chinese medicines and external drugs can be actively helped to improve the infrastructure of the physiotherapy department, and help the physiotherapy department to improve the theory and diagnosis and treatment level. During the domestic training, TCM doctors should do a good job in the prevention and treatment of common diseases in physiotherapy department, such as spinal disease, stroke, arthritis, facial paralysis and other diseases in both Chinese and English. 2024 Vol. 21 (1): 143-147 [Abstract] ( 239 ) [HTML 1KB] [PDF 2053KB] ( 1742 ) Nursing Science 148 WANG Yingying, BAO Shasha, DENG Jinying, CHEN Yanfei, LIU Xiaoqing, XIAO Ning Effects of self-disclosure nursing intervention on perceptive social support, anxiety and coping styles in maintenance hemodialysis patients Objective To explore the effects of self-disclosure nursing intervention on social support, anxiety and coping style of maintenance hemodialysis patients. Methods A total of 94 patients with maintenance hemodialysis were divided into control group and intervention group, 47 patients in each group. The control group received routine nursing, and the intervention group implemented self-disclosure intervention on the basis of the control group. The level of self-disclosure, perceptive social support, anxiety and coping style scores before and after intervention were compared between the two groups. Results Before intervention, there were no significant differences in the scores of self-disclosure, understanding of social support, anxiety and positive coping styles between the two groups. After 6 weeks of intervention, the scores of self-disclosure, perceptive social support and coping style in the intervention group were higher than those in the control group (P<0.05), and the scores of anxiety and negative coping style were lower than those in the control group (P<0.05). Conclusions Self-disclosure intervention can improve the self-disclosure ability, understanding social support level and positive coping level of MHD patients, and improve the anxiety of MHD patients. 2024 Vol. 21 (1): 148-153 [Abstract] ( 290 ) [HTML 1KB] [PDF 2004KB] ( 1951 ) 154 WU Wenya, HE Caiyun Correlational study on post-thyroidectomy symptom distress and quality of life among thyroid cancer patients Objective To investigate the degree of symptom distress in patients with thyroid cancer after operation and analyze its correlation with quality of life. Method One hundred and twenty-seven patients with thyroid cancer who visited a tertiary care hospital in Hunan Province from October 2022 to April 2023 were selected as the study subjects and investigated with general data questionnaire, Anderson Symptom Scale-Thyroid Cancer Module, and thyroid cancer-specific quality of life scale. Results The total score of symptom distress was (91.95±38.57) points and the total score of thyroid cancer-specific quality of life scale was (41.71±8.12) points. Multivariate analysis showed that gender, surgical methods and quality of life were the influencing factors of symptom distress in patients with thyroid cancer (P<0.01), and symptom distress was positively correlated with quality of life (r=0.599, P<0.001). Conclusion Patients with thyroid cancer after operation generally have symptom distress and relatively low quality of life. Medical staff can take corresponding measures to reduce the symptom distress of patients after operation, so as to improve the quality of life of patients. 2024 Vol. 21 (1): 154-158 [Abstract] ( 316 ) [HTML 1KB] [PDF 1949KB] ( 1797 ) 159 YUAN Tian, LIU Lihua, LI Lijun The Current Situation and Influencing Factors of Disaster Preparedness Among Intern Nursing Students in the Context of Emergency Public Health Events. Objective To understand the current situation of disaster preparedness and related influencing factors of nursing interns in the context of public health emergencies,and to provide a theoretical basis for improving the disaster nursing ability of nursing interns. Method The researchers used a self-made general data questionnaire,a Chinese version of the disaster preparedness assessment tool and a psychological resilience assessment tool to survey 273 intern nursing students in four tertiary hospitals in Changsha,Hunan Province in the form of paper questionnaires and online questionnaires. Results The disaster preparedness score of 273 intern nursing students was（192.17±48.90）,and the average score of the entries was（4.27±1.08）,and the disaster preparedness was medium. Among them,the educational background of the intern nursing students,whether they have studied professional disaster nursing courses in school,whether the hospital where the internship is located has held disaster nursing training and psychological resilience are significant influencing factors of disaster preparedness. Conclusion The disaster preparedness of intern nursing students under the background of public health emergencies is at a medium level. It is necessary to implement and promote the improvement of the academic qualifications of intern nursing students,strengthen the education of professional disaster nursing courses,carry out disaster nursing training to improve the disaster preparedness of intern nursing students,and give play to their role as a disaster rescue reserve force. 2024 Vol. 21 (1): 159-163 [Abstract] ( 284 ) [HTML 1KB] [PDF 1937KB] ( 2143 ) 164 CHEN Ting, CHEN Yan, CHEN Yefan, LUO Yaoyue Construction of Indicator System for Perioperative Nursing Service Needs of Liver Transplantation Patients Objective To construct an indicator system for perioperative nursing service needs of liver transplantation patients, providing a reference for the perioperative nursing needs of liver transplantation patients. Methods Based on the thematic panel discussion, literature review, and clinical practice, a draft of indicators for perioperative nursing service needs of liver transplantation patients was developed. This draft underwent two rounds of expert consultations involving 18 experts from relevant fields such as Hepatobiliary Surgery and Gastroenterology. The indicators were revised based on expert consultations, and the weights of the indicators were calculated using the Analytic Hierarchy Process (AHP). Results The constructed indicator system for perioperative nursing service needs of liver transplantation patients includes three primary indicators (preoperative nursing, intraoperative nursing, and postoperative nursing), 13 secondary indicators, and 45 tertiary indicators. The positive coefficient of the two rounds of expert consultations was 100%, and the expert authority coefficients were 0.880 and 0.868, while the expert coordination coefficients were 0.252 and 0.261. This indicator system covers various aspects including preoperative nursing assessment, preoperative education, psychological support, intraoperative anesthesia monitoring and assistance, postoperative monitoring, infection prevention, and pain management, providing a scientific basis for the perioperative nursing service needs of liver transplantation patients. Conclusion The constructed indicator system for perioperative nursing service needs of liver transplantation patients is scientifically reliable and can serve as a reference for the perioperative nursing care of liver transplantation patients. 2024 Vol. 21 (1): 164-163 [Abstract] ( 249 ) [HTML 1KB] [PDF 2030KB] ( 1718 )

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