基于IL-18、ZO-1探讨针刺及艾灸对腹泻型肠易激综合征大鼠模型的影响

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Abstract Objective To explore the effect of acupuncture and moxibustion on the expression of zonula occludens-1(ZO-1), and blood interleukin-18(IL-18) and other related inflammatory factors in the intestinal tissues of rats with irritable bowel syndrome with predominant diarrhea(IBS-D). Methods Ten rats were randomly selected from 58 male SD rats as a control group. The remaining 48 were used to prepare the IBS-D model by acetic acid enema combined with restraint tail-clamping stress method. The remaining 48 rats were prepared by using acetic acid enema combined with the restraint tail-clamping stress method.40 rats that were successfully modeled were randomly divided into the model group, acupuncture group, moxibustion group, and western medicine group, with 10 rats in each group. In the acupuncture group, acupuncture was performed at the “foot-sanli” for 15 min each time, and the needle was twisted every 5 min for 20 s. In the moxibustion group, moxibustion was performed at the “foot-sanli” for 15 min each time, and in the western medicine group, pivoxyl bromide was administered by gastric lavage for 14 d. In the modeling group, the IBS-D models were randomly divided into the model group, moxibustion group and western medicine group, each of which had 10 animals. In the western medicine group, the rats were gavaged with pivacurium bromide tablets (Deshotep) for 14 d. Observations were made on the fecal character score and diarrhea index of the rats at the end of the modeling and the intervention, and the serum IL-18 level and the expression of ZO-1 in the colon tissues of the rats were analyzed and statistically analyzed at the end of the intervention. Results After modeling, the fecal character score and diarrhea index of rats in the other four groups were elevated compared with the control group. After intervention, fecal character score and diarrhea index of rats in the model group were elevated compared with those in the control group; fecal character score and diarrhea index of rats in the acupuncture, moxibustion, and western medicine groups were reduced compared with those in the model group, and fecal character score of rats in the acupuncture group was lower than that of rats in the western medicine group; ZO-1 expression in the colonic tissues of rats in the control group, acupuncture, moxibustion, and Western medicine (Pivacidium bromide ) ZO-1 expression was elevated to different degrees in rat colon tissues; compared with the model group, the serum IL-18 concentration values in the acupuncture group and control group were lower than those in the model group, and the IL-18 concentration levels in the moxibustion group and western medicine group were significantly lower than that in the model group. Conclusion Both acupuncture and moxibustion improved diarrhea symptoms in IBS-D model rats, and the mechanism may be achieved by regulating IL-18 levels, increasing ZO-1 protein expression, remodeling the tight junction structure, decreasing its tissue intercellular permeability, and repairing the damaged intestinal barrier.

Key words： irritable bowel syndrome with predominant diarrhea acupuncture moxibustion zonula occludens-1 interleukin-18

Received: 16 July 2024

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R245

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	HE Yongjia
	LAI Biyu
	HONG Mengying
	CHEN Yao
	LI Xinwu
	ZHANG Peiqin
	ZHANG Shuiqing
	SHE Chang

Cite this article:

HE Yongjia,LAI Biyu,HONG Mengying等. Exploring the effects of acupuncture and moxibustion on a rat model of diarrhea-type irritable bowel syndrome based on IL-18 and ZO-1[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(5): 1-5.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I5/1

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