甘草酸通过抑制GSDMD介导的非典型细胞焦亡改善铜绿假单胞菌诱导角膜炎症和损伤

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Abstract Objective To investigate how glycyrrhetinic acid improves inflammation and corneal injury induced by Pseudomonas aeruginosa by inhibiting Gasdermin-D (GSDMD) mediated atypical cell pyroptosis. Methods Prepare a rat model of Pseudomonas aeruginosa keratitis and randomly divide the rats into a control group, PA group, and GLY+PA group. Compare the keratitis index of each group; Enzyme linked immunosorbent assay (ELISA) was used to detect serum inflammatory factors; Hematoxylin eosin (HE) staining was used to detect pathological changes in corneal tissue; Immunofluorescence detection showed positive expression of caspase-11 and GSDMD in corneal tissue for cysteine; Protein blotting and qRT PCR were used to detect the expression of caspase-4, caspase-5, caspase-11, GSDMD protein, and mRNA in corneal tissue. Results The keratitis index and serum levels of interleukin-1β (IL-1 β) (78.54±8.03) and interleukin-18(IL-18) (26.31±2.74), inflammatory cell density, percentage of positive expression areas of caspase-11 and GSDMD, as well as the expression of caspase-4, caspase-5, caspase-11, GSDMD protein and mRNA in corneal tissue of rats in the PA group were significantly higher than those in the control group; Compared with the PA group, the GLY+PA group significantly reduced the keratitis index and serum levels of IL-1β (46.27±5.11) and IL-18(13.52±1.46), inflammatory cell density, percentage of positive expression areas of caspase-11 and GSDMD, as well as the expression of caspase-4, caspase-5, caspase-11, GSDMD protein and mRNA in corneal tissue. Conclusion Glycyrrhizic acid inhibits the corneal inflammatory response and tissue damage in rats with Pseudomonas aeruginosa keratitis, and its mechanism of action may be related to the inhibition of GSDMD-mediated activation of the atypical cellular pyroptosis pathway.

Key words： glycyrrhizic acid gasdermin-D mediated atypical cellular pyroptosis Pseudomonas aeruginosa inflammation corneal damage

Received: 27 March 2024

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	TANG Ying
	LI Pengfei
	YU Jinjin

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TANG Ying,LI Pengfei,YU Jinjin. Glycyrrhetinic acid improves inflammation and corneal injury induced by Pseudomonas aeruginosa by inhibiting GSDMD mediated atypical cell pyroptosis[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(4): 18-23.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I4/18

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