毛冬青提取物对小鼠放射性口腔黏膜炎模型创面修复作用研究

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Abstract Objective To investigate the effect of Ilex pubescens extract on the wound healing effect of mice with radiation oral mucositis and to explore its possible mechanism. Methods Male C3H mice with 8 weeks old were selected to reproduce the model of radiation oral mucositis（RTOM）in mice by X-ray irradiation of the oral and tongue parts. They were randomly divided into model group,positive control and experimental groups. HE staining was used to observe the recovery of mucosal lesions and the density of fibroblasts. The expression of IL-6 and TNF-α protein was detected by immunohistochemistry. Results The mouse RTOM model was successfully replicated. After 7 days,the model group mice showed grade III oral ulcer,thinning of the oral mucosal epithelial layer,slight disorder of keratinized epithelial structure,dilation of capillaries,and visible infiltration of inflammatory cells;The mice in the cell growth factor group exhibited grade II oral ulcer,slightly increased thickness of buccal mucosal epithelium compared to the normal group,and normal keratinized epithelial structure;After 7 days of treatment,the mice in the Ilex exract group showed grade I oral ulcer,increased keratinization of the buccal mucosa,and normal keratinized epithelial structure. Immunohistochemical and Western blot results showed that the expression of TNF - and IL-6 in the cheek mucosa of mice in the cell growth factor group and Ilex exract group was significantly lower than that in the model group. The expression of TNF - and IL-6 in the cheek mucosa of mice in the Ilex exract group was significantly lower than that in the cell growth factor group. The positive rates of TNF - and IL-6 expression in the cell growth factor group and Ilex exract group were significantly lower than those in the model group. The positive rates of TNF - and IL-6 expression in the Ilex exract group were significantly lower than those in the cell growth factor group. Conclusion Extracts of Ilex have a repairing effect on ulcer wounds in mice with RTOM.

Key words： mice RTOM model mice Ilex extract inflammatory factor

Received: 30 October 2023

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	LIU Quan
	WEI Hongxia

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LIU Quan,WEI Hongxia. Research on the effect of Ilex pubescens extract on wound repair of mice with radiation oral mucositis model[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(1): 90-95.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I1/90

[1] RAO S,HEGDE SK,RAO P,et al.Honey Mitigates Radiation-Induced Oral Mucositis in Head and Neck Cancer Patients without Affecting the Tumor Response[J]. Foods,2017,6(9):77.
[2] 邢志伟,姜恩海,江波,等. GBZ162《放射性口腔炎诊断标准》的编制说明[J]. 中国辐射卫生,2007,16(3):280-281.
[3] TROTTI A,BELLM L A,EPSTEIN J B,et al.Mucositis incidence,severity and associated outcomes in patients with head and neck cancer receiving radiotherapy with or without chemotherapy:a systematic literature review[J]. Radiother Oncol,2003,66(3):253-262.
[4] POPOVTZER A,EISBRUCH A.Advances in radiation therapy of head and neck cancer[J]. Expert Rev Anticancer Ther,2008,8(4):633-644.
[5] DAMKIER P,KAPLAN YC,SHECHTMAN S,et al.Ondansetron in pregnancy revisited:Assessment and pregnancy labelling by the European Medicines Agency(EMA)& Pharmacovigilance Risk Assessment Committee(PRAC)[J]. Basic Clin Pharmacol Toxicol,2021,128(4):579-582.
[6] 姚敏娜,舒任庚,李韦韦,等. 大叶冬青叶的化学成分及其抗氧化活性研究[J]. 中国药师,2018,21(11):1895-1897.
[7] 陈颖,王存琴,汪雷,等. 大叶冬青叶中8种活性成分提取工艺研究[J]. 皖南医学院学报,2019,38(1):21-24.
[8] 张悦,李陆军,张鹏,等. 冬青属两种植物的抗炎作用及自由基清除能力研究[J]. 华中科技大学学报(医学版),2011,40(1):71-74.
[9] 钟海森,覃骊兰,杜正彩,等. 壮药毛冬青的应用概况[J]. 河北中医,2018,40(5):793-797.
[10] 李丽华,李文辉,崔建国,等. 放疗所致急性口腔黏膜炎治疗观察[J]. 齐鲁医学杂志,2003,18(4):434.
[11] SONIS ST.New thoughts on the initiation of mucositis[J]. Oral Dis,2010,16(7):597-600.
[12] 陈晨,于然,陈冬梅,等. 放化疗性口腔黏膜炎动物模型的研究进展与比较[J]. 海南医学院学报,2022,28(15):1188-1193.
[13] 邹晓龙,陈媛,王艳,等. 放化疗性口腔黏膜炎动物模型研究进展[J]. 口腔疾病防治,2020,28(5):322-326.
[14] 王怡娟,王松灵. 放化疗性口腔黏膜炎模型建立及防治现状[J]. 口腔生物医学,2012,3(3):151-154.
[15] 韦彩丽. 冬青叶在治疗化疗引起口腔溃疡患者中的应用[J]. 护理实践与研究,2014,11(7):151-152.
[16] NOWACKI LC,STECHMAN-NETO J,SCHIEFER EM,et al.Ilex paraguariensis extract as drugs alternative for pain[J]. Acta Pharm,2020,71(3):383-398.
[17] 周中流,尹文清,何淑君,等. 毛冬青中三萜皂苷类化合物的提取分离及电喷雾多级质谱快速分析研究[J]. 天然产物研究与开发,2013,25(3):307-311.
[18] 董克臣,张萌,梁毅,等. 西黄胶囊对大鼠放射性口腔黏膜炎的防护作用[J]. 现代肿瘤医学,2020,28(3):383-386.
[19] 牛艳红,冯崇锦,黄伟安,等,依达拉奉预防大鼠放射性口腔黏膜炎的初步研究[J]. 中华口腔医学研究杂志(电子版),2010,4(4):332-337.