右美托咪定对内毒素急性肺损伤小鼠炎症反应的影响

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Abstract Objective Effect of dexmedetomidine (DEX) on endotoxin induced acute lung injury in mice. Methods 32 healthy male C57BL/6 mice were randomly divided into 4 groups (8 mice/group), sham operation group (control group), endotoxin acute lung injury group (LPS group), high concentration DEX group (HD group), low concentration DEX group (LD group). Control group was injected with saline of equal volume; LPS group was intraperitoneally injected with LPS 3 mg/kg; In DEX+LPS group, DEX (40 μg/kg or 20 μg/kg). The inflammatory changes of lung tissue and the ratio of wet weight to dry weight (W/D) were measured by pathological sections; Detection of serum IL-6, IL-8 and IL-1β by enzyme-linked immunosorbent assay (ELISA) Change of content; The changes of miR-155 and miR-146a in lung tissue were detected by qRT-PCR. Results Compared with control group, LPS group and DEX group had significant lung inflammation, lung W/D ratio, IL-6, IL-8, and IL-1β the content and expression level of miR-155 increased, while the expression level of miR-146a decreased (^*P<0.05); Compared with LPS group, lung inflammation, lung W/D ratio, IL-6, IL-8, and IL-1β in DEX group were significantly improved the content and expression level of miR-155 decreased, while the expression level of miR-146a increased (^#P<0.05); Compared with LD group, lung inflammation, lung W/D ratio, IL-6, IL-8, and IL-1β in HD group were significantly improved the content and expression level of miR-155 decreased, while the expression level of miR-146a increased (^$P<0.05). Conclusion Dextrmetomidine (DEX) can reduce the inflammatory reaction in mice with acute lung injury induced by endotoxin, which may be related to miR-146a and miR-155.

Key words： demetomidine acute lung injury caused by endotoxin miR-146a miR-155

Received: 29 January 2023

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R363

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	Articles by authors
	CHEN Qiuxia
	LIU Liu
	CHEN Guimei
	CHEN Anji
	DUAN Boqing

Cite this article:

CHEN Qiuxia,LIU Liu,CHEN Guimei等. Effect of dexmedetomidine on inflammatory response in mice with acute lung injury induced by endotoxin[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(3): 24-27.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I3/24

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