CircRNA_0629在胆囊癌患者中的临床意义及其与预后的关系

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Abstract Objective: To determine the clinical significance of circular RNA 0629(circRNA_0629) in patients with gallbladder cancer (GBC) and its relationship with prognosis. Methods The results of GSE100363 and GSE121714 microarrays were analyzed using bioinformatics to screen for circRNAs that might be associated with GBC metastasis. Cancer tissues and paracancerous tissues from 110 GBC patients were collected, and the expression levels of circRNAs were verified by quantitative real-time PCR (qRT-PCR). The relationship between circRNAs expression and clinicopathological characteristics was analyzed by chi-square test. The association between circRNAs expression and overall survival (OS) was analyzed by Kaplan-Meyer (K-M) method and Long-Rank test. The factors that affecting prognosis of GBC were analyzed by Cox proportional risk regression model. The area under the curve (AUC) values of circRNAs for the diagnosis of GBC liver metastases and TNM staging were assessed by receiver operating characteristic (ROC) curves. Results A total of three circRNAs were screened, including circRNA_049637, circRNA_402458 and circRNA_0629. The expression of circRNA_0629 was higher in cancer tissues than that in paracancerous tissues. The differences of circRNA_049637 and circRNA_402458 expression in cancer tissues were not statistically significant when compared with paracancerous tissues. The expression of circRNA_0629 was correlated with liver metastasis and TNM staging. The 1- and 2-year OS were lower in GBC patients with high circRNA_0629 expression than those of GBC patients with low circRNA_0629 expression. High circRNA_0629 expression, liver metastasis and stage III~IV were independent risk factors for poor prognosis in GBC patients. circRNA_0629 had high diagnostic values for GBC liver metastases and TNM staging (AUC: 0.929 and 0.835). Conclusions circRNA_0629 expression is upregulated in GBC and is an independent risk factor for poor prognosis. circRNA_0629 expression may serve as a novel biomarker to assist in the evaluation of liver metastasis and TNM staging in GBC.

Key words： circRNA_0629 GBC OS liver metastasis biomarker

Received: 18 January 2023

PACS:

R735.8

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	LI Jibin
	JIANG Shihao
	YANG Zhen
	ZHOU Ning

Cite this article:

LI Jibin,JIANG Shihao,YANG Zhen等. Clinical significance of circRNA_0629 in patients with gallbladder cancer and its relationship with prognosis[J]. journal1, 2023, 20(2): 93-100.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I2/93

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