circRHOT1靶向miR-144-3p调控喉癌细胞增殖、迁移和凋亡

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Abstract Objective To analyze the expression levels of circRHOT1 and miR-144-3p in laryngeal cancer tissues, and further to explore the effect of circRHOT1 targeting miR-144-3p on the biological behavior of laryngeal cancer cells. Methods circRHOT1 and miR-144-3p expression in 32 specimens of laryngeal cancer and 15 specimens of vocal cord polyp tissues was estimated by RT-qPCR. Pearson correlation analysis was used to determined the expression relationship of circRHOT1 and miR-144-3p in laryngeal cancer tissues. si-NC, si-circRHOT1, miR-NC, miR-144-3p mimics, si-circRHOT1+anti-miR-NC, si-circRHOT1+anti-miR-144-3p were respectively transfected into TU686 cells. CCK-8 method, scratch healing assays and flow cytometry were applied to analyze the proliferation, migration ability and apoptosis of TU686 cell in vitro. Dual luciferase reporter assay was used to analyze the targeting relationship between circRHOT1 and miR-144-3p. Results The expression level of circRHOT1 in laryngeal cancer tissue was significantly higher than that in vocal cord polyp tissues, while the expression level of miR-144-3p was significantly lower than that in vocal cord polyp tissues. The expression of circRHOT1 and miR-144-3p in laryngeal cancer tissues was negatively correlated. After down-regulating the expression of circRHOT1, the inhibition rate, apoptosis rate and miR-144-3p expression level of TU686 cells were significantly increased, and the scratch healing rate was significantly reduced. After overexpression of miR-144-3p, the inhibition rate and apoptosis rate of TU686 cells were significantly increased, and the scratch healing rate was significantly reduced. miR-144-3p was considered as a direct target of circRHOT1. Inhibition of miR-144-3p could attenuate the effect of circRHOT1 down-regulation on the proliferation, apoptosis and migration of TU686 cells. Conclusion Down-regulation of circRHOT1 could induce laryngeal cancer cell apoptosis and inhibit their proliferation and migration by targeting and up-regulating miR-144-3p expression.

Key words： laryngeal carcinoma circRHOT1 miR-144-3p cell proliferation migration apoptosis

Received: 06 September 2022

PACS:

R739.6

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	Articles by authors
	WANG Xuyan
	ZHANG Pei
	WANG Lili
	HAN Mingming
	ZHANG Jing
	ZHAO Mingxin

Cite this article:

WANG Xuyan,ZHANG Pei,WANG Lili等. circRHOT1 targets miR-144-3p to regulate the proliferation, migration and apoptosis of laryngeal cancer cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(2): 63-69.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I2/63

[1] 李梦婷, 夏立军, 李锦荣. 晚期喉癌的治疗进展[J]. 中国耳鼻咽喉颅底外科杂志, 2020, 26(3): 343-348.
[2] Marur S, Forastiere AA.Head and Neck Squamous Cell Carcinoma: Update on Epidemiology, Diagnosis, and Treatment[J]. Mayo Clin Proc, 2016, 91(3): 386-396.
[3] 王丽萍, 魏永祥, 葛林虎. 环状RNA与肿瘤研究进展[J]. 中华肿瘤防治杂志, 2017, 24(20): 1480-1486.
[4] Wang L, Long H, Zheng Q, et al.Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression[J]. Mol Cancer, 2019, 18(1): 119-129.
[5] Qu S, Hao X, Song W, et al.Circular RNA circRHOT1 is upregulated and promotes cell proliferation and invasion in pancreatic cancer[J]. Epigenomics, 2019, 11(1): 53-63.
[6] Zhang SY, Lu ZM, Lin YF, et al.miR-144-3p, a tumor suppressive microRNA targeting ETS-1 in laryngeal squamous cell carcinoma[J]. Oncotarget, 2016, 7(10): 11637-11650.
[7] Zang Y, Li J, Wan B, et al.circRNA circ-CCND1 promotes the proliferation of laryngeal squamous cell carcinoma through elevating CCND1 expression via interacting with HuR and miR-646[J]. J Cell Mol Med, 2020, 24(4): 2423-2433.
[8] Chu YL.Circ_0067934 correlates with poor prognosis and promotes laryngeal squamous cell cancer progression by sponging miR-1324[J]. Eur Rev Med Pharmacol Sci, 2020, 24(8): 4320-4327.
[9] Tian L, Cao J, Jiao H, et al.CircRASSF2 promotes laryngeal squamous cell carcinoma progression by regulating the miR-302b-3p/IGF-1R axis[J]. Clin Sci (Lond), 2019, 133(9): 1053-1066.
[10] Ling S, He Y, Li X, et al.CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR-125a-3p[J]. J Cell Mol Med, 2020, 24(17): 9881-9889.
[11] Zhang H, Ge Z, Wang Z, et al.Circular RNA RHOT1 promotes progression and inhibits ferroptosis via mir-106a-5p/STAT3 axis in breast cancer[J]. Aging (Albany NY), 2021, 13(6): 8115-8126.
[12] Zhang Q, Cheng F, Zhang Z, et al.Propofol suppresses non-small cell lung cancer tumorigenesis by regulation of circ-RHOT1/miR-326/FOXM1 axis[J]. Life Sci, 2021, 27(1): 119042-119052.
[13] Mo BY, Pang JS, Dai WB, et al.A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma[J]. Comput Math Methods Med, 2021, 2021(1): 6684186-6684196.
[14] Wu J, Zhao Y, Li F, et al.MiR-144-3p: a novel tumor suppressor targeting MAPK6 in cervical cancer[J]. J Physiol Biochem, 2019, 75(2): 143-152.
[15] Chen YJ, Guo YN, Shi K, et al.Down-regulation of microRNA-144-3p and its clinical value in non-small cell lung cancer: a comprehensive analysis based on microarray, miRNA-sequencing, and quantitative real-time PCR data[J]. Respir Res, 2019, 20(1): 48-58.
[16] Tian F, Wang H, Ma H, et al.miR-144-3p inhibits the proliferation, migration and angiogenesis of multiple myeloma cells by targeting myocyte enhancer factor 2A[J]. Int J Mol Med, 2020, 46(3): 1155-1165.
[17] He L, Liao L, Du L. miR-144-3p inhibits tumor cell growth and invasion in oral squamous cell carcinoma through the downregulation of the oncogenic gene, EZH2[J]. Int J Mol Med, 2020, 46(2): 828-838.
[18] Liu F, Zhang J, Qin L, et al.Circular RNA EIF6(Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation[J]. Aging (Albany NY), 2018, 10(12): 3806-3820.