Objective Investigate the composition and influencing factors of hospitalization expenses of gallstone patients. Method Epidata 3.1 was used for data double entry, and SPSS 18 software was used for statistical analysis. Results The average hospitalization days of patients with cholecystolithiasis were 11.43±3.24 days, The cost of patients is mainly composed of medicine expenses, operation fees and auxiliary examination fees, among them, expenses for drugs, surgery, and auxiliary examinations accounted for 42.71%, 18.72% and 12.61% respectively of hospital expenses. Conclusion The cost of hospitalization is mainly composed of medical expenses, examination fee and operation fee. The factors influencing hospitalization expenses include actual hospitalization days, postoperative complications, times of rescue and nursing level.