Abstract：Objective To explore the effect and mechanism of SCD1 protein expression on the ability of ferroptosis activator RSL3 to induce ferroptosis in bladder cancer cells. Method T24 cells were treated with SCD1 small interfering RNA (siRNA), specific SCD1 inhibitor (A9) or combined with RSL3; The effects of inhibiting SCD1 expression or combining with RSL3 on cell proliferation and clonogenesis were detected by CCK-8 and clonogenesis assay; Malondialdehyde (MDA) and lipid peroxides (LPO) were determined to investigate the effect of down-regulated SCD1 on the ability of RSL3 to induce ferroptosis in bladder cancer cells T24; Finally, glutathione peroxidase 4(GPX4) expression was detected by western blot. Results Both siRNA silencing and A9 treatment decreased the proliferation and colony formation ability of bladder cancer cells. Compared with the single treatment group, The two SCD1 inhibition methods combined with RSL3 further increased the MDA and LPO content in the cells, and enhanced the degree of inhibition on the proliferation and clonal colony formation of bladder cancer cells. In addition, inhibition of SCD1 enhanced the inhibitory effect of RSL3 on GPX4 protein expression. Conclusion Down-regulation of SCD1 can inhibit the growth of bladder cancer cells by enhancing the inhibition of GPX4 protein expression by RSL3 and inducing ferroptosis.
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