基于网络药理学探讨参菊洗剂治疗阴道炎的作用机制

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摘要 目的: 基于网络药理学方法分析参菊洗剂治疗阴道炎的作用机制。方法: 通过中药系统药理学分析平台（TCMSP）对参菊洗剂的活性成分和靶点进行筛选;利用GeneCards数据库获取阴道炎靶点。利用R语言将药物与疾病靶点进行并集分析,获得疾病与药物共同靶点信息。将共同靶点导入Cytoscape3.8.2,构建“药物-疾病-靶点”网络图,再利用STRING平台构建蛋白质相互作用网络。最后利用R语言对共同靶点进行基因本体（GO）分析和基因相互作用（KEGG）通路分析。结果: 通过筛选得到,参菊洗剂有效活性成分112个,参菊洗剂-阴道炎的共同靶点83个,主要通过调控RELA、MAPK3、MYC、HIF1A、MAPK1、TP53等靶基因,进而影响IL-17、HIF-1和PI3K-Akt等信号通路,最终发挥治疗阴道炎作用。结论: 通过网络药理学方法得出参菊洗剂可通过多成分、多靶点、多通路发挥对阴道炎的治疗作用。

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	刘晓亭
	江志超

关键词 ：参菊洗剂, 阴道炎, 网络药理学

Abstract：Objective To explore the mechanism of Ginseng Lotion in the treatment of vaginitis by network pharmacology, so as to provide a theoretical basis for later experimental research. Methods The active ingredients and their targets of ginseng lotion Decoction were screened by the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), vaginitis targets were obtained using GeneCards database. The R language is used to obtain a target set for the treatment of vaginitis by Ginseng Lotion. The target set uses to construct “drug-disease-target” network and the Protein-Protein interaction (PPI) network to exPlore its mechanism. Finally, Functional enrichment analysis of gene ontology (GO) and Pathway enrichment analysis based on the Kyoto EncycloPedia of Genes and Genomes (KEGG) were Performed using R language. Results There were 112 active comPounds and 83 targets related to the treatment of vaginitis in Ginseng Lotion. The key targets involved RELA、MAPK3、MYC、HIF1A、MAPK1、TP53, etc. It mainly involves TNF signaling pathway, IL-17 signaling pathway, HIF-1 signaling pathway and PI3K-Akt signaling pathway to Play a theraPeutic role in stomach cancer. Conclusion Through network pharmacology methods, it is concluded that Ginseng Lotion can treat vaginitis through multi-component, multi-target and multi-pathway.

Key words： Ginseng Lotion vaginitis network Pharmacology

收稿日期: 2022-10-12

中图分类号:

R961

通讯作者: 江志超,E-mail：602618074@qq.com

引用本文:

刘晓亭, 江志超. 基于网络药理学探讨参菊洗剂治疗阴道炎的作用机制[J]. 湖南师范大学学报(医学版), 2023, 20(1): 85-91. LIU Xiaoting, JIANG Zhichao. Mechanism on the Ginseng Lotion In the treatment of vaginitis Based on Network Pharmacology. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(1): 85-91.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I1/85

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